Wa09 h9 hes cell line

Manufactured by WiCell

Sourced in United States

The WA09 (H9) hES cell line is a well-characterized human embryonic stem cell line derived from a donated blastocyst. It is a commonly used cell line in research and has been extensively studied and characterized.

Automatically generated - may contain errors

Lab products found in correlation

Matrigel, by BD (2 mentions) Mtesr1, by STEMCELL (2 mentions) A1113803, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by American Type Culture Collection (1 mentions) Trypsin, by Thermo Fisher Scientific (1 mentions) Puromycin, by Thermo Fisher Scientific (1 mentions) Crl 4000, by American Type Culture Collection (1 mentions) Str profiling, by American Type Culture Collection (1 mentions) Relesr, by STEMCELL (1 mentions)

Close spelling variants of this product

H9/WA09 (33 citations) H9 line (8 citations) H9 cells (6 citations) WA09 line (2 citations)

2 protocols using wa09 h9 hes cell line

hES Cell Maintenance in mTeSR1 on Matrigel

Check if the same lab product or an alternative is used in the 5 most similar protocols

WA09 (H9) hES cell line was purchased from WiCell (Wisconsin) and maintained in mTeSR1 (05850, StemCell Technologies) on growth factor reduced Matrigel (354230, BD). Cells were passaged every 3 days using 0.5% EDTA in PBS.

Wolff S.C., Kedziora K.M., Dumitru R., Dungee C.D., Zikry T.M., Beltran A.S., Haggerty R.A., Cheng J., Redick M.A, & Purvis J.E. (2018). Inheritance of OCT4 predetermines fate choice in human embryonic stem cells. Molecular Systems Biology, 14(9), e8140.

+ Open protocol

+ Expand

Culturing hTERT-RPE, U2OS, and H9 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

hTERT retinal pigment epithelial cells (RPE) were obtained from the ATCC (ATCC® CRL‐4000™) and cultured in DMEM supplemented with 10% fetal calf serum (FBS) and penicillin/streptomycin. U2OS cells were obtained from the laboratory of Dr. Yue Xiong and cultured in DMEM supplemented with 10% fetal bovine serum (FBS) and penicillin/streptomycin (Gibco). WA09 (H9) hES cell line was purchased from WiCell (Wisconsin) and maintained in mTeSR1 (85850, StemCell Technologies) on growth factor reduced Matrigel (354230, BD). Cells were authenticated by STR profiling (ATCC, Manassas, VA) and confirmed to be free of mycoplasma. Cells were passaged using trypsin (25300054, Gibco) for RPE and U2OS or ReLeSR™ (05872, StemCell Technologies) for H9 as needed. When required, the medium was supplemented with selective antibiotics (2 μg/ml puromycin for RPE and U2OS; 0.5 μg/ml puromycin for H9; A1113803, Gibco).

Chao H.X., Fakhreddin R.I., Shimerov H.K., Kedziora K.M., Kumar R.J., Perez J., Limas J.C., Grant G.D., Cook J.G., Gupta G.P, & Purvis J.E. (2019). Evidence that the human cell cycle is a series of uncoupled, memoryless phases. Molecular Systems Biology, 15(3), e8604.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!