1H and 13C NMR spectra were recorded at 25 °C on the Brucker Avance III 400 MHz. Unless otherwise specified, chemical shifts δ were expressed in parts per million (ppm) based on the residual solvent signal in DMSO-d6 (δ 2.50 ppm) or tetramethylsilane (TMS) in CDCl3 (δ 0.00 ppm), and coupling constants J are given in Hz. Coupling patterns are abbreviated as s (singlet), d (doublet), t (triplet), q (quartet), quin (quintet), ABq (AB quartet), and m (multiplet). Electrospray mass spectra were recorded on a Waters LCT Premier mass spectrometer in positive ion mode. Reverse phase HPLC was performed using Waters HPLC system equipped with Waters-1525 solvent delivery systems and Waters 2998 UV detector. Separation was achieved on a semi-preparative Phenomenex (Torrance, CA) Jupiter® 5μm C18 300Å column (5 micron, 250 × 10.00 mm, Part Number: 00G-4053-N0) at a flow rate maintained at 4.0 mL/min. A solvent system composed of water containing 0.1% TFA as solvent A and acetonitrile containing 0.1% TFA as solvent B was used over the stated gradient. The column effluent was monitored with a UV/Vis detector set at 760 nm.
Waters 2998 uv detector
Manufactured by Waters Corporation
Sourced in United States
The Waters 2998 UV detector is a laboratory instrument used for the detection and quantification of analytes in liquid chromatography. It measures the absorbance of ultraviolet light by the sample, which can be used to identify and quantify the compounds present.
Automatically generated - may contain errors
Lab products found in correlation
Hplc system, by Waters Corporation (1 mentions)
Lct premier mass spectrometer, by Waters Corporation (1 mentions)
Avance 3 400 mhz, by Brucker (1 mentions)
Xevo g2 xs qtof mass spectrometer, by Waters Corporation (1 mentions)
Avance 600 spectrometer, by Bruker (1 mentions)
C18 column, by YMC (1 mentions)
Silica gel g plates, by Qingdao Marine Chemical (1 mentions)
Precoated silica gel 60 f254 plates, by Merck Group (1 mentions)
Av 600 spectrometer, by Bruker (1 mentions)
Nicolet is10 ft ir spectrometer, by Thermo Fisher Scientific (1 mentions)
Lcms it tof, by Shimadzu (1 mentions)
4 protocols using waters 2998 uv detector
1
Spectroscopic Characterization of Compounds
2
PthII Release from HKUST-1 Carrier
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The release curve of PthII@HKUST-1 was determined according to the method described in literature [23 (link)]. PthII@HKUST-1 powder was dispersed in PBS, transferred it to a dialysis bag after being ultrasonically dispersed, and put the dialysis bag into the deionized water. The release system was placed in a shaking box (150 rpm) for incubation. At different time points, the precipitate in the dialysis bag was collected by centrifugation, eluted with ethanol ultrasonic for three times, and centrifuged to collect the supernatant, and then the release amount of PthII in PthII@HKUST-1 was detected by high performance liquid chromatography (HPLC, Waters 2998 UV detector) after filtration with 0.22 µm filter membrane. The liquid chromatography detection conditions are as follows: mobile phases were acetonitrile (A) and 0.1% formic acid in water (B), gradient elution conditions: 0–10 min was 60–80% A, 10–10.1 min was 80–100% A, 10.1–15 min was 100% A,15–15.1 min was 100–60% A, 15.1–20 min was 60% A, with a flow rate 0.3 mL/min, injection volume 1 µL, column temperature 40 °C. The maximum absorption wavelength of PthII is 228 nm.
3
Structural Characterization of Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The HR-ESI-MS spectra were performed on Waters Xevo G2-XS QTOF mass spectrometer (Waters, Milford, MA, USA). NMR spectra were measured on a Bruker Avance-600 spectrometer (Bruker, Karlsruhe, Germany) in DMSO-D6 operating at 600 MHz for 1H NMR, HMBC and HMQC, 150 MHz for 13C NMR, respectively. Tetramethylsilane (TMS) was used as internal standard. Column chromatography was performed with silica gel (200–300 mesh) purchased from Qingdao Ocean Chemical Group Co. Ltd (Qingdao, China). Thin-layer chromatography was conducted on silica gel G plates (Qingdao Marine Chemical Inc.). Semi-preparative HPLC with 1525 binary pump combined Waters 2998 UV detector (Waters Co., Milford, MA, USA.) and YMC C18 column (5 μm, 20 mm × 10 mm; YMC Co., Ltd., Japan) were also used to isolate the compounds. Methanol (MeOH) and acetonitrile (ACN) were HPLC grade (Fisher, USA). Purified water was purchased from YiBao Co, Ltd (Shenzhen, China). Other solvents used were analytical grade (Beijing Chemical Works, Beijing, China).
4
Advanced Analytical Techniques for Chemical Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mass spectrometry (MS) data were collected using liquid chromatography-mass spectrometry ion trap time-of-flight (LCMS-IT-TOF) (Shimadzu, Kyoto, Japan). Nuclear magnetic resonance (NMR) experiments were recorded using a Bruker AV-600 spectrometer (Bruker Biospin, Rheinstetten, Germany). Optical rotations were recorded on a Rudolph Autopol IV (Rudolph Research, Hackettstown, NJ, USA). Infrared (IR) spectra were obtained on Nicolet™ iS™ 10 FTIR spectrometer (Thermo Fisher Scientific, Waltham, MA, USA). Reverse phase Ultra performance liquid chromatography (UPLC) was performed using a Waters Arc2695 UPLC system (Waters, Milford, Fairfield Country, SC, USA) with a Waters CORTECS TM C18 (4.6 × 50 mm, 2.7 µm) column, equipped with a Waters 2998 UV detector. Column chromatography was performed using silica gel (100–200 mesh, 200–300 mesh, Qingdao Oceanic Chemicals, Qingdao, China). Pre-coated silica gel 60 F254 plates (Merck, Darmstadt, Germany) were used for thin-layer chromatography (TCL). Semi-preparative HPLC was carried out on a Waters 1525 pump with a Waters 2998 PDA detector equipped with a Waters XBridge® BEH C18 OBDTM prep column (10 × 150 mm, 5 µm, Waters, Milford, MA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!