Angelica polysaccharide (AP) was purchased from JRDUN Biotechnology Co. Ltd. (Shanghai, China); DMEM and fetal bovine serum (FBS) were purchased from Gibco. Co. (NY, USA); Cell Counting Kit-8 (CCK-8) was purchased from Dojindo Biochem (Shanghai, China); antidinitrophenol (DNP) IgE and DNP-HAS were purchased from Sigma-Aldrich (MO, USA); Fluo-3 AM reagents were purchased from Life Tech. Co. (CA, USA); rats histamine, IL-1, TNF-α, IL-6, LTC4, β-hexosaminidase, and MCP-1/CCL2 ELISA kits were purchased from the Boster Co. (Wuhan, China); p-Fyn and Fyn primary antibodies were purchased from Abcam Co. (Cambridge, UK); p-Akt, Akt, p-P38, P38, and NF-κB p65 primary antibodies were purchased from CST Co. (MA, USA); TNF-α, IL-4, and GAPDH primary antibodies were purchased from Santa Cruz Biotech. (CA, USA); BCA protein kit and horseradish peroxidase- (HPR-) conjugated secondary antibodies were purchased from Beyotime Co. (Jiangsu, China); PVDF membrane was purchased from Millipore Biotech. (MA, USA).
Anti dinitrophenol dnp ige
Manufactured by Merck Group
Sourced in China
The Anti-dinitrophenol (DNP) IgE is a laboratory equipment product used for the detection and quantification of IgE antibodies specific to dinitrophenol. It is designed to assist in the diagnosis and monitoring of allergic reactions associated with dinitrophenol exposure.
Automatically generated - may contain errors
Lab products found in correlation
Human ige, by Merck Group (2 mentions)
Anti ige, by Euromedex (2 mentions)
Bca protein kit, by Beyotime (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Dnp has, by Merck Group (1 mentions)
3 protocols using anti dinitrophenol dnp ige
1
Angelica Polysaccharide Bioactivity Evaluation
2
Mast Cell Activation Assay
3
Mast Cell Activation Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Human mast cells were incubated in culture medium with or without human
IgE (1 μg/mL; Sigma-Aldrich) overnight at 37°C. BMCMCs were
incubated with or without anti-dinitrophenol (DNP) IgE (0.5 μg/mL; Sigma
Aldrich) overnight at 37°C. The cells were then washed and distributed in
96-well flat-bottom plates at a density of 105 cells in 50 μL
of Tyrode’s buffer at 37°C. 40 minutes later, cells were treated
with 50 μL of prewarmed stimuli diluted in Tyrode’s buffer for 45
minutes at 37°C. As a positive control of functional activation, human
mast cells were stimulated with anti-IgE (10 ng/mL; Euromedex) and BMCMCs with
DNP-BSA (10 ng/mL; Sigma Aldrich). To assess whether AD antigens could activate
mast cells, human mast cells and BMCMCs were stimulated with increasing
concentrations of D. farinae and SEB (5 ng/mL, 50 ng/mL and 500
ng/mL), alone or together. β-Hexosaminidase release in the supernatants
was measured as previously described25 (link).
IgE (1 μg/mL; Sigma-Aldrich) overnight at 37°C. BMCMCs were
incubated with or without anti-dinitrophenol (DNP) IgE (0.5 μg/mL; Sigma
Aldrich) overnight at 37°C. The cells were then washed and distributed in
96-well flat-bottom plates at a density of 105 cells in 50 μL
of Tyrode’s buffer at 37°C. 40 minutes later, cells were treated
with 50 μL of prewarmed stimuli diluted in Tyrode’s buffer for 45
minutes at 37°C. As a positive control of functional activation, human
mast cells were stimulated with anti-IgE (10 ng/mL; Euromedex) and BMCMCs with
DNP-BSA (10 ng/mL; Sigma Aldrich). To assess whether AD antigens could activate
mast cells, human mast cells and BMCMCs were stimulated with increasing
concentrations of D. farinae and SEB (5 ng/mL, 50 ng/mL and 500
ng/mL), alone or together. β-Hexosaminidase release in the supernatants
was measured as previously described25 (link).
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