Orca er 1394 digital camera

One of the mCherry constructs and p35S-GFP-DcNMCP1HT (see the “Plasmid preparation” subsection) (0.5 μg each) were mixed, and co-introduced into celery (Apium graveolens) epidermal cells using the Biolistic PDS-1000/He particle delivery system (Bio-Rad) as described previously.²⁵ (link) Cells were incubated for 24 h at room temperature after being transformed, and signals were observed using the BX50 epifluorescence microscope (Olympus) equipped with the ORCA-ER-1394 digital camera (Hamamatsu Photonics, Hamamatsu, Japan). The fluorescence mirror units U-MGFPHQ and U-MWIG2 (Olympus) were used to image GFP and mCherry, respectively. Images were processed with GIMP and Inkscape.

The CDSs of ATB''δ (AT5G28900.1) and FASS (AT5G18580.1) were amplified by PCR using primers listed in Supplementary Table S2 and the cDNA sample prepared as described above. The PCR products were digested with KpnI and SpeI, and inserted into the KpnI–SpeI site of pBS-35SMCS-cYFP (Tsugama et al., 2012c (link)), generating pBS-35S-ATB''δ-cYFP and pBS-35S-FASS-cYFP. The CDS of VIP1 was inserted into the pBS-35SMCS-nYFP-2 vector as previously described (Tsugama et al., 2012c (link), 2014 (link)), generating pBS-35S-VIP1-nYFP. Either pBS-35SMCS-nYFP-2 or pBS-35S-VIP1-nYFP (500 ng) was mixed with pBS-35SMCS-cYFP, pBS-35S-ATB''δ-cYFP, or pBS-35S-FASS-cYFP (500 ng), and bound to gold particles. These constructs were co-introduced into onion epidermal cells with the Biolistic PDS-1000/He particle delivery system (Bio-Rad, Hercules, CA, USA). Cells were then incubated for 12 h at room temperature, and BiFC signals were observed using the BX50 epifluorescence microscope (Olympus) equipped with the fluorescence mirror units U-MGFPHQ (Olympus) (for detecting BiFC signals) and an ORCA-ER-1394 digital camera (Hamamatsu Photonics, Hamamatsu, Japan). Images were processed with GIMP and Inkscape.