The following staining procedures were performed on a random 3-μm paraffin-embedded liver sections: Haematoxylin–Eosin (H&E), Cleaved-Caspase 3 (9661S, Cell Signalling) staining as an apoptosis marker, von Willebrand Factor (vWF) (IR527, DAKO) for endothelial cell activation. Ki-67 staining as proliferation marker in bile ducts (IR626, DAKO). Phosphohistone 3 (pH3) staining as a mitosis marker (M-06-570-3KL, Milipore). CD68+ (Cluster of Differentiation 68+) staining for liver macrophages (M0876, DAKO). Cytokeratin 7 staining for bile ducts (DAKO). Periodic acid Schiff (PAS) staining was performed for qualitative analysis of glycogen content.
Cytokeratin 7 staining for bile ducts
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Cytokeratin 7 is a protein used as a marker for staining bile ducts in laboratory settings. It is a specific and reliable tool for identifying and visualizing bile duct structures.
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2 protocols using cytokeratin 7 staining for bile ducts
1
Comprehensive Liver Histopathology Profiling
2
Multimodal Liver Tissue Analysis
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The following staining procedures were performed on random 3-µm-thick paraffin-embedded liver sections: hematoxylin and eosin, cleaved caspase 3 (9661S, Cell Signaling) staining as an apoptosis marker, von Willebrand Factor (IR527, DAKO) for endothelial cell activation, Ki-67 staining as a proliferation marker in bile ducts (IR626, DAKO), pH3 staining as a mitosis marker (M-06-570-3KL, Milipore), CD68 staining for liver macrophages (M0876, DAKO) and cytokeratin 7 staining for bile ducts (DAKO). PAS staining was performed for qualitative analysis of glycogen content.
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