780 rd infrared dye

Western blot analysis of experimental and control cell lysates was carried out using the Odyssey^® Infrared System (LI-COR Biosciences, Lincoln, NE) and polyclonal anti-phospho-AMPK-α (Thr 172) antibody (Cat # 07-681SP), polyclonal Anti-p21 antibody (Cat # 0506550), and polyclonal Anti-Kip1 (p27) (Cat # 060445) were purchased from Millipore (Millipore Corporation, Iselin, NJ ). Polyclonal beta actin antibody (Cat # sc-1615) and polyclonal RASSF1C antibody (sc-18724) were purchased from Santa Cruz Biotechnology, Inc (Santa Cruz, CA), and fluorescently-labeled secondary antibodies IRDye^® 680 and 780 RD Infrared Dye were purchased from LI-COR (LI-COR Biosciences, Lincoln, NE). The experiments were repeated at least 3 times. Protein levels were normalized to actin levels (the loading control).

Western blot analysis of experimental and control cell lysates was carried out using the Odyssey^® Infrared System (LI-COR Biosciences, Lincoln, NE, USA). Cell lysate from control and experimental cells were prepared using RIPA lysis buffer supplemented with 1× protease inhibitors (Sigma) and 25 μg of cell lysates were used to run Western blots. β-catenin, vimentin, and snail polyclonal antibodies were purchased from Cell Signaling Technology, Inc. Polyclonal beta actin antibody (Cat # sc-1615) was purchased from Santa Cruz Biotechnology, Inc (Santa Cruz, CA, USA), and fluorescently-labeled secondary antibodies IRDye^® 680 and 780 RD Infrared Dye were purchased from LI-COR (LI-COR Biosciences, Lincoln, NE, USA). The experiments were repeated at least 3 times. Protein levels were normalized to actin levels (the loading control).

Immunoblots were performed using gene-specific antibodies. Western blot analysis of experimental and control cell lysates was carried out using the Odyssey^® Infrared System (LI-COR Biosciences, Lincoln, NE, USA). Cell lysate from control and experimental cells was prepared using RIPA lysis buffer supplemented with 1X protease inhibitors (Sigma, St. Louis, MO, USA) and 25 µg of cell lysates was used to run Western blots. P4HA2 (Cat #HPA027824) antibody was purchased from Sigma Inc (Sigma, St. Louis, MO, USA). PLOD2 (Cat # 50-557-311) was purchased from Fisher Scientific (Fisher Scientific, Pittsburgh, PA, USA). Collagen I (Cat # 1310-01) antibody was purchased from Southern Biotech (Birmingham, AL, USA). Polyclonal beta actin antibody (Cat # sc-1615) was purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA), and fluorescently labeled secondary antibodies IRDye^® 680 and 780 RD Infrared Dye were purchased from LI-COR (LI-COR Biosciences, Lincoln, NE, USA). The experiments were repeated at least 3 times. Protein levels were normalized to actin levels (loading control). The average intensities of signals on Western blot images were quantified and normalized using the Odyssey image analysis software.