Mg500

Manufactured by Merck Group

Sourced in France

The MG500 is a laboratory equipment product from Merck Group. It is designed for general laboratory use. The MG500 features precise temperature control and advanced monitoring capabilities to support various experimental and analytical applications.

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Lab products found in correlation

Gs500, by Merck Group (4 mentions) Shandon cytospin 4, by Thermo Fisher Scientific (1 mentions) May gr nwald, by Merck Group (1 mentions) Eclipse ti s inverted microscope, by Nikon (1 mentions) Shandon cytospin, by Thermo Fisher Scientific (1 mentions) Dm2000 inverted microscope, by Leica camera (1 mentions) Cm1850 uv, by Leica camera (1 mentions) Stemi 508, by Zeiss (1 mentions)

5 protocols using mg500

Validation of Cell Sorting Gates

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Overall, 10⁵ of total and sorted cells according to α4-integrin and Band 3 surface expression as previously described [23 (link)] were cytospun using the Thermo Scientific Shandon 4 Cytospin to validate the sorting gates. The slides were stained with May-Grünwald (Sigma Aldrich, MG500, St Quentin Fallavier, France) solution for 5 min, with May-Grünwald solution diluted by half for another 5 min, and subsequently stained with Giemsa solution (Sigma Aldrich, GS500, St Quentin Fallavier, France) diluted 10 times for 15 min. Cells were imaged using a Nikon Eclipse Ti-S inverted microscope with a 40 × /0.6 Plan Fluor objective.

Moras M., Hattab C., Gonzalez-Menendez P., Martino S., Larghero J., Le Van Kim C., Kinet S., Taylor N., Lefevre S.D, & Ostuni M.A. (2020). Downregulation of Mitochondrial TSPO Inhibits Mitophagy and Reduces Enucleation During Human Terminal Erythropoiesis. International Journal of Molecular Sciences, 21(23), 9066.

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Cell Cytospin and Differential Staining

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Briefly, 1 × 10⁵ cells in 200 μL were cytospun onto coated slides using the Thermo Scientific Shandon Cytospin. The slides were stained with May-Grünwald (Sigma MG500) solution for 5 minutes, rinsed in 40 mM Tris buffer (pH 7.2) for 90 seconds, and subsequently stained with Giemsa solution (Sigma GS500) for 15 minutes. The cells were imaged by using a Leica DM2000 inverted microscope.

An C., Huang Y., Li M., Xue F., Nie D., Zhao H., Chen L., Yazdanbakhsh K., Sun L., Jiang Z., Mohandas N, & An X. (2021). Vesicular formation regulated by ERK/MAPK pathway mediates human erythroblast enucleation. Blood Advances, 5(22), 4648-4661.

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May-Grünwald-Giemsa Staining Protocol

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Sorted cells were diluted in 250μl iscove’s modified delbecco medium (IMDM) containing 20% FBS and cytospun on glass slides using the Shandon Cytospin 2. The slides were stained with May-Grünwald (Sigma MG500) solution for 5 minutes, rinsed in 25 mM Tris buffer (pH 7.4) for 1.5 minutes, and then stained with a 1:20 diluted Giemsa solution (Sigma GS500) for 20 minutes. The cells were imaged in bright field using Evos microscope.

Hasan S., Mosier M.J., Conrad P., Szilagyi A., Gamelli R.L, & Muthumalaiappan K. (2018). Terminal Maturation of Orthochromatic Erythroblasts Is Impaired in Burn Patients. Journal of Burn Care & Research: Official Publication of the American Burn Association, 39(2), 286-294.

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Bone Marrow Cell Cytospin and Staining

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Approximately 2 × 10⁵ total bone marrow cells were diluted in 250μl iscove’s modified delbecco medium (IMDM) containing 20% fetal bovine serum and cytospun on glass slides using the Shandon Cytospin 2. The slides were stained with May-Grünwald (Sigma MG500) solution for 5 minutes, rinsed in 25 mM Tris buffer (pH 7.4) for 1.5 minutes, and then stained with a 1:20 diluted Giemsa solution (Sigma GS500) for 20 minutes. The cells were imaged in bright field using Evos microscope.

Hasan S., Mosier M.J., Szilagyi A., Gamelli R.L, & Muthumalaiappan K. (2017). Discrete beta-adrenergic mechanisms regulate early and late erythropoiesis in Epo-resistant anemia. Surgery, 162(4), 901-916.

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Neurological Tissue Preparation for Histological Analysis

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After the last neural recording, birds were overdosed with the ketamine/xylazine solution (1:1 ketamine 10 mg/mL + xylazine 2 mg/mL). Electrolytic lesions were made at the recording sites by applying a high-voltage current to the tetrodes for 10 s to 15 s. Then, the birds were perfused intracardially with the phosphate buffer (phosphate-buffered saline; 0.1 mol, pH = 7.4, 0.9% sodium chloride, 5 °C) followed by 4% paraformaldehyde (PFA). Brains were incubated for at least 2 d in PFA and a further 2 d in 30% sucrose solution in PFA. Coronal 60-μm brain sections were cut at −20 °C using a cryostat (Leica CM1850 UV), mounted on glass slides, stained with the Giemsa dye (MG500, Sigma-Aldrich), and coverslipped with Eukitt (FLUKA). Brain sections were examined under the stereomicroscope (Stemi 508, Carl Zeiss) to estimate the anatomical position of recording sites.

Kobylkov D., Mayer U., Zanon M, & Vallortigara G. (2022). Number neurons in the nidopallium of young domestic chicks. Proceedings of the National Academy of Sciences of the United States of America, 119(32), e2201039119.

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