Pdlim2

Immunohistochemical stainings of 20 primary cHL, 10 follicular lymphoma, 15 diffuse large B-cell lymphoma and 10 Burkitt lymphoma cases were performed as previously described.^{33 (link)} Primary antibodies (PDLIM2, PA5-31484, Thermo Scientific, Darmstadt, Germany; PDLIM2, ab68220, Abcam; IκBα, sc-371, Santa Cruz) were applied in a dilution of 1:100 (PDLIM2 antibodies) or 1:500 (IκBα) overnight.

Cells were lysed in RIPA buffer consisting of (150 mM NaCl, 1% Triton-X, 0.5% Sodium deoxycholate, 0.1% SDS and 50 mM Tris pH 8.0) before protein concentration was determined using a colorimetric BCA protein assay (Pierce), and immunoblotting proceeded as described previously (Kaempchen et al., 2003 (link)). Samples intended for MS measurement were separated using 4–15% gradient pre-cast gels (Bio-rad). The antibodies used in the study included: Merlin (1:1000), pMerlin (1:500), HDAC1 (1:1000) and PDLIM2 (1:500) from Cell Signaling Technology; PDLIM2 (1:500) from Santa Cruz Biotechnology and GAPDH (1:50.000) from Millipore.