L glutamine without sodium bicarbonate

RPMI-1640 medium with L-glutamine without sodium bicarbonate (Sigma) was prepared in DI water (15–20 ℃) and 34.53 gr of MOPS (Sigma) was added. The final pH was adjusted with NaOH to a pH of 7 and added additional water to 1 l of the final volume solution. A stock solution of extracted SLs produced by S. riodocensis in dimethyl sulfoxide (DMSO, Sigma) was prepared and kept at −20 °C until use. Hyphal growth assay was formed in 5 ml of RPMI-1640 medium and RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) [18 (link)]. Cell suspension was diluted at 1 × 10⁶ cells ml⁻¹ in medium and incubated with different concentrations of purified SLs (0 μg ml⁻¹, 8 μg ml⁻¹, 16 μg ml⁻¹, 32 μg ml⁻¹, 62.5 μg ml⁻¹, 125 μg ml⁻¹, 250 μg ml⁻¹, 500 μg ml⁻¹, and 1000 μg ml⁻¹) at 37 °C with agitation at 200 rpm for 5 h. Aliquots of samples were visualized under a bright-field using a 40X objective lens by Huma Scope Premium microscope and photographed.

Wild type strains of C. albicans SC531418 (link), C.
glabrata CG46218 (link), C. tropicalis MYA340450 (link) and C. lusitaniae CL618 (link), were used in this
study. Frozen glycerol stock of the strain was regularly revived on YPD agar
medium (1% Bacto yeast extract, 2% Bacto peptone, 2% glucose and 2% Bacto agar).
For broth culture, strain was grown in YPD medium at 30 °C with
agitation (200 rpm). RPMI-1640 medium with L-glutamine without sodium
bicarbonate (Sigma) was buffered with 0.165 M morpholinepropanesulfonic
acid (Sigma) to a pH of 7. Stock solutions of extracted SL (supplementary material), AmB (Sigma) and FLZ
(Sigma) were prepared in dimethyl sulfoxide (DMSO, Sigma), and stored at
−20 °C until use.