Cells were grown in SILAC MEM medium (Thermo Fisher Scientific) lacking lysine and arginine, supplemented with dialysed 10% FBS (Sigma-Aldrich), 200 mg/l light L-Proline (Sigma-Aldrich) and either heavy L-Lysine (13C615N2; 146 mg/l) and L-Arginine (13C615N4; 84 mg/l) (Cambridge Isotope Laboratories) or their light equivalents (Sigma-Aldrich). Cells were passaged with non-enzymatic Gibco Cell Dissociation Buffer (Thermo Fisher Scientific). >95% heavy amino acid incorporation rate was validated by mass-spectrometry.
Silac mem medium
Manufactured by Thermo Fisher Scientific
SILAC MEM medium is a cell culture medium designed for performing stable isotope labeling with amino acids in cell culture (SILAC) experiments. It provides the essential amino acids, vitamins, and other nutrients required for cell growth and metabolism. The medium is specially formulated to enable the incorporation of stable isotope-labeled amino acids into cellular proteins, which is a key requirement for SILAC-based proteomics studies.
Automatically generated - may contain errors
2 protocols using silac mem medium
1
SILAC Metabolic Labeling Protocol
2
SILAC Metabolic Labeling Protocol
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!