Log In Sign up
The largest database of trusted experimental protocols

Nylons

Manufactured by BD
Visit Supplier

Nylons are a type of synthetic fiber used in various laboratory equipment. They are known for their durability, flexibility, and resistance to chemicals and heat. Nylons are commonly used in the manufacture of equipment components, such as seals, gaskets, and tubing, to ensure reliable performance in laboratory settings.

Automatically generated - may contain errors

Lab products found in correlation

Celltrace violet cell proliferation kit, by Thermo Fisher Scientific (2 mentions) Cytochalasin d, by Merck Group (2 mentions)

Close spelling variants of this product

70 µm nylon strainer 40-µm nylon filter 70 μm pore size nylon cell strainer 70-μm nylon cell strainer 35-μm nylon mesh cell strainer 100- and 40-mm nylon cell strainers 70 µm nylon filter Pore size nylon mesh strainer 70 µm nylon sieves Nylon 100 μm mesh filter

2 protocols using nylons

1

Efferocytosis and Macrophage Activation Assay

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
BMDMs and PCMs were generated and stimulated as described above. To generate apoptotic cells (ACs), thymii from C57BL/6J mice were harvested and mechanistically dissociated, filtered on 100μm nylons (Falcon), pelleted and resuspended in RPMI medium supplemented with 10% FBS. Apoptosis was induced by UV exposure at 312nm for 10min and cells were maintained in culture for an additional 2 hours. This method results in 70-90% apoptosis23 (link). ACs were labelled with CellTrace™ Violet Cell Proliferation kit (ThermoFisher) according to the manufacturer’s instructions. Fluorescent ACs were washed twice with PBS before use. For one round efferocytosis: stained ACs were added at a 5:1 ratio on plated macrophages for 45min. For two rounds efferocytosis: unlabelled ACs were added at a 5:1 ratio on plated macrophages for 45min. Cells were then washed 3 times and macrophages were incubated for 1h. Stained ACs were then added at a 5:1 ratio on macrophages for 45min. Cells were washed 3 times and macrophages were stained and analysed for AC content and activation markers by flow cytometry. In some experiment, macrophages were treated for 15 min prior efferocytosis with cytochalasin D (5μM, Sigma). For Seahorse extracellular flux analysis ACs were injected directly, before drug treatment, during the assay.
Merlin J., Ivanov S., Dumont A., Sergushichev A., Gall J., Stunault M., Ayrault M., Vaillant N., Castiglione A., Swain A., Orange F., Gallerand A., Berton T., Martin J.C., Carobbio S., Masson J., Gaisler-Salomon I., Maechler P., Rayport S., Sluimer J.C., Biessen E.A., Guinamard R.R., Gautier E.L., Thorp E.B., Artyomov M.N, & Yvan-Charvet L. (2021). Non-canonical glutamine transamination sustains efferocytosis by coupling redox buffering to oxidative phosphorylation. Nature metabolism, 3(10), 1313-1326.
+ Open protocol
+ Expand
2

Efferocytosis and Macrophage Activation Assay

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
BMDMs and PCMs were generated and stimulated as described above. To generate apoptotic cells (ACs), thymii from C57BL/6J mice were harvested and mechanistically dissociated, filtered on 100μm nylons (Falcon), pelleted and resuspended in RPMI medium supplemented with 10% FBS. Apoptosis was induced by UV exposure at 312nm for 10min and cells were maintained in culture for an additional 2 hours. This method results in 70-90% apoptosis23 (link). ACs were labelled with CellTrace™ Violet Cell Proliferation kit (ThermoFisher) according to the manufacturer’s instructions. Fluorescent ACs were washed twice with PBS before use. For one round efferocytosis: stained ACs were added at a 5:1 ratio on plated macrophages for 45min. For two rounds efferocytosis: unlabelled ACs were added at a 5:1 ratio on plated macrophages for 45min. Cells were then washed 3 times and macrophages were incubated for 1h. Stained ACs were then added at a 5:1 ratio on macrophages for 45min. Cells were washed 3 times and macrophages were stained and analysed for AC content and activation markers by flow cytometry. In some experiment, macrophages were treated for 15 min prior efferocytosis with cytochalasin D (5μM, Sigma). For Seahorse extracellular flux analysis ACs were injected directly, before drug treatment, during the assay.
Merlin J., Ivanov S., Dumont A., Sergushichev A., Gall J., Stunault M., Ayrault M., Vaillant N., Castiglione A., Swain A., Orange F., Gallerand A., Berton T., Martin J.C., Carobbio S., Masson J., Gaisler-Salomon I., Maechler P., Rayport S., Sluimer J.C., Biessen E.A., Guinamard R.R., Gautier E.L., Thorp E.B., Artyomov M.N, & Yvan-Charvet L. (2021). Non-canonical glutamine transamination sustains efferocytosis by coupling redox buffering to oxidative phosphorylation. Nature metabolism, 3(10), 1313-1326.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!