chloride trihydrate (HAuCl4·3H2O), dithiothreitol
(DTT), and triethylamine
(TEA) were purchased from J&K Scientific LTD (Peking, China).
All oligonucleotides (
and purified through HPLC by Sangon Biotech Co., Ltd. (Shanghai, China).
NAP-5 columns (Sephadex G-25 Medium, DNA grade) were purchased from
Pharmacia Biotech (GE Healthcare Bio-Sciences, Uppsala, Sweden). ZEN0117-disposable
low volume cuvettes (100 μL) were purchased from Malvern Instruments
Ltd. (Malvern, England). Millipore H2O purified with a
Millipore ultrapure water system was used throughout the experiments.
The bare AuNPs (13.0 ± 0.4 nm in diameter measured by transmission
electron microscope,
prepared following the literature protocols.41 (link) Through the immobilization process, the polydispersity of the DNA/AuNP
conjugates was all in the range from 0.17 to 0.28, indicating their
good quality and narrow distribution for the cumulants analysis.