LRTI was defined by clinical signs or symptoms of respiratory tract infection and pulmonary infiltrate on radiographic imaging compatible with a bacterial or viral pneumonia (eg, consolidation, interstitial infiltrate, or ground-glass opacities). Healthcare visits and hospitalizations were classified as URI-related if URI symptoms were addressed in the encounter, regardless of the primary reason for the encounter. Peak flu season was defined as December through March [21 ]. Nasal swabs were submitted for respiratory viral testing at the clinicians’ discretion. Testing was generally performed using a laboratory-developed multiplex polymerase chain reaction (PCR) test that can detect 12 respiratory viruses [22–24 (link)]. Additional tests, including the Biofire FilmArray Respiratory Panel or Influenza-specific tests (Cepheid Xpert Flu), were also captured. Clostridioides difficile testing was performed using PCR (Xpert C. difficile; Cepheid).
Filmarray respiratory panel
Manufactured by Cepheid
The FilmArray Respiratory Panel is a multiplex PCR-based test that detects the presence of multiple respiratory pathogens, including viruses and bacteria, in a single patient sample. The core function of the FilmArray Respiratory Panel is to provide a comprehensive diagnostic solution for the rapid identification of respiratory infections.
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Lab products found in correlation
2 protocols using filmarray respiratory panel
1
Respiratory Illness Diagnosis and Characterization
2
Pooled Nasopharyngeal Specimens for SARS-CoV-2 Testing
Check if the same lab product or an alternative is used in the 5 most similar protocols
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RPN pools were prepared by mixing aliquots from nasopharyngeal specimens in viral transport medium from patients with respiratory symptoms that previously tested negative for routine respiratory pathogens using multiplex diagnostic panels (e.g., BioMerieux FilmArray Respiratory Panel, Cepheid Xpert® Xpress Flu/RSV). RPN specimens (n = 3040) collected at MSHS hospitals and outpatient facilities between 30 December 2019 and 7 March 2020 were organized into groups of ten, and stored at −80 °C. Notably, these specimens had not previously been tested for SARS-CoV-2. The RPN pools (n = 304) were prepared in an isolated class II biological safety cabinet at a separate location from the Clinical Microbiology and research labs that had never been used for handling respiratory specimens or viruses.
Briefly, 400 µL of viral transport medium from each specimen was manually aliquoted one at a time into a sterile 5 mL snap-cap centrifuge tube (ASi, C2520). Once each specimen was aliquoted, the 4 mL volume was mixed manually by pipetting and 600 μL aliquots were reserved for SARS-CoV-2 NAAT. RPN specimens and pools were stored at −80 °C.
Briefly, 400 µL of viral transport medium from each specimen was manually aliquoted one at a time into a sterile 5 mL snap-cap centrifuge tube (ASi, C2520). Once each specimen was aliquoted, the 4 mL volume was mixed manually by pipetting and 600 μL aliquots were reserved for SARS-CoV-2 NAAT. RPN specimens and pools were stored at −80 °C.
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