Cells were seeded in complete medium in 10 cm fibronectin-coated tissue cultures dishes at a density of 5,000 cells/cm2 and allowed to reach confluence. Cells received either a 0 Gy (control) or 10 Gy (treatment) dose of radiation. Media was replaced with fresh complete media and cells incubated for a further 48 h. Cells were washed with serum-free medium and serum starved for 4 h prior to stimulation with a growth factor mixture consisting of VEGF-A (50 ng/mL for 5 min), VEGF-C (200 ng/mL for 10 min) or VEGF-D (200 ng/mL for 10 min), EGF [100 ng/mL for 5 min, PromoCell (Germany)], bFGF [100 ng/mL for 5 min, (PromoCell, Germany)], IGF-1 [100 nM for 5 min, (PromoCell, Germany)] and heparin (1 μg/mL for 5 min; added 5 min before the addition of bFGF). Cells were lysed and analysed with the human Phospho RTK-array kit (R&D Systems, United States) as per manufacturer’s instructions.
Igf 1
Manufactured by PromoCell
Sourced in Germany
IGF-1 is a protein hormone that plays a crucial role in cell growth, proliferation, and differentiation. It is produced primarily by the liver and is essential for normal growth and development. IGF-1 is often used in laboratory research to study its effects on various cell types and biological processes.
Automatically generated - may contain errors
Lab products found in correlation
Basic fibroblast growth factor (bfgf), by PromoCell (2 mentions)
Epidermal growth factor (egf), by PromoCell (2 mentions)
Human phospho rtk array kit, by R&D Systems (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
2 protocols using igf 1
1
Growth Factor Signaling in Irradiated Cells
2
Primary HDLECs Culture Conditions
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Primary HDLECs from juvenile foreskin were purchased from PromoCell (Cat No. c-12216; Germany) and cultured in MV2 medium containing 10 ng/ml bFGF, 5 ng/ml EGF, and 20 ng/ml IGF-1 (PromoCell). HEK293T human embryonic kidney cells were cultured in Dulbecco’s Modified Eagle’s Medium (Gibco BRL, USA) supplemented with 10% fetal bovine serum and antibiotics (100 U/ml penicillin and 100 μg/ml streptomycin; Gibco BRL). All cells were maintained at 37°C in the presence of 5% CO2.
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