Total proteins were extracted from sperm under different treatments with lysis buffer. The protein concentration was measured with a BCA assay kit (TaKaRa, Dalian, China). The proteins were then separated by 12.5% SDS-PAGE and transferred to a PVDF membrane. The membrane was blocked with 5% BSA for 2 h and then incubated with anti-cleave caspase3 (CST, 1:1,000), anti-cleave caspase 9 (CST, 1:1,000), anti-p53 (CST, 1:1,000), anti-Parp-1 (CST, 1:1,000), anti-CPT1 (Santa Cruz,1:1,000), anti-ACADVL (Santa Cruz,1:1,000), and anti-α-tubulin (Santa Cruz,1:1,000) at 4 °C overnight. HRP conjugated goat anti-rabbit and goat anti-mouse antibodies were used as the secondary antibody, respectively (1:2000 final dilution). The reagent for enhanced chemiluminescence (Bio-Rad) was used for detection and developed by X-ray film (Champchemi Top610, China).
Anti cpt1
Manufactured by Santa Cruz Biotechnology
Sourced in United Kingdom
Anti-CPT1 is a laboratory reagent used for the detection and quantification of Carnitine Palmitoyltransferase I (CPT1), an enzyme involved in the transport of long-chain fatty acids into the mitochondria for beta-oxidation. The product can be used in various biochemical and molecular biology applications to study the regulation and activity of CPT1.
Automatically generated - may contain errors
Lab products found in correlation
Anti α tubulin, by Santa Cruz Biotechnology (1 mentions)
Bca assay kit, by Takara Bio (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Chemidoc xr, by Bio-Rad (1 mentions)
Ripa buffer, by Beyotime (1 mentions)
Anti p acc, by Cell Signaling Technology (1 mentions)
Anti acc, by Cell Signaling Technology (1 mentions)
Anti myod, by Santa Cruz Biotechnology (1 mentions)
Image lab system, by Bio-Rad (1 mentions)
Anti myhc, by Abcam (1 mentions)
Anti oct4, by Santa Cruz Biotechnology (1 mentions)
β actin, by Sungene Biotech (1 mentions)
2 protocols using anti cpt1
1
Sperm Protein Extraction and Analysis
2
Protein Expression Analysis in Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total protein was extracted using radioimmunoprecipitation assay (RIPA) buffer (Beyotime, Shanghai, China). A total of 20 μg of protein was separated by SDS-PAGE and transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, Boston, MA, USA). Membranes were then incubated overnight with primary antibodies as follows: anti-MYOD (Santa Cruz, Dallas, TX, USA), anti-MYOG (Novus Biologicals, CO, USA), anti-MyHC (Abcam, Cambridge, UK), anti-OCT4 (Santa Cruz), anti-ELOVL6 (Cell Signaling Technology), anti-ACC (Cell Signaling Technology), anti-p-ACC (Cell Signaling Technology), and anti-CPT1 (Santa Cruz Biotechnology). The next day, the membranes were washed three times with Tris-buffered saline containing Tween 20 (TBST). Then, secondary antibody was added and the samples were incubated for 1 h at 4°C with shaking. Secondary antibody was β-actin (Sungene Biotech, Tianjin, China). Protein bands were detected using ChemiDOC XRS+ and the Image Lab system (Bio-Rad, USA)
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