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Kbro3

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KBrO3 is a chemical compound with the formula KBrO3. It is a potassium salt of bromic acid. KBrO3 is commonly used in various laboratory applications.

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Lab products found in correlation

Voso4 h2o, by Merck Group (1 mentions) 13c6 d glucose, by Cambridge Isotopes (1 mentions) 15n l tyrosine, by Cambridge Isotopes (1 mentions) 3 3 13c2 cystine, by Cambridge Isotopes (1 mentions) 15n choline chloride, by Cambridge Isotopes (1 mentions) 2 deoxyguanosine 15n2 13c10 5 monophosphate, by Cambridge Isotopes (1 mentions) Milli q, by Merck Group (1 mentions) Sodium chloride (nacl), by Avantor (1 mentions) Ca no3 2, by Merck Group (1 mentions) Caso4, by Merck Group (1 mentions) Caco3, by Thermo Fisher Scientific (1 mentions) Mg no3 2, by Merck Group (1 mentions) Mgcl2, by Merck Group (1 mentions) Nano3, by Merck Group (1 mentions) L cysteine, by Merck Group (1 mentions) Cacl2, by Merck Group (1 mentions) L glutathione reduced, by Merck Group (1 mentions)

6 protocols using kbro3

1

Protective Effects of Vitamin C Against KBrO3 Toxicity

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KBrO3 used was purchased from Sigma Chemicals (Sigma, St Louis, MO, USA), while the vitamin C was in the form of Cevarol oral drops (CID, Cairo, Egypt).
KBrO3 was injected (IP) (groups 3 and 4) at a toxic dose of 20 mg/kg body weight/dose twice weekly for 4 weeks prepared as 40 mg/ml of distilled water [14 (link)]. The vitamin C was given orally (groups 2 and 4) in a high dose of 30mg/kg/day; the dose was divided twice daily [3 ], for 4 weeks concomitant with KBrO3.
El-Deeb M.E, & Abd-El-Hafez A.A. (2015). Can vitamin C affect the KBrO3 induced oxidative stress on left ventricular myocardium of adult male albino rats? A histological and immunohistochemical study. Journal of Microscopy and Ultrastructure, 3(3), 120-136.
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2

MARVELD1 Genotype Potassium Bromate Exposure

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1500 ppM KBrO3 (Sigma-Aldrich, USA) was given to 8 months old MARVELD1+/+ mice, MARVELD1+/− mice, and MARVELD1−/− mice in their drinking water for 5 weeks. After 5 weeks, all the mice were sacrificed. The collected blood serum, part of the livers and brains frozen quickly in liquid nitrogen for ELISA assays. The remaining livers and brains were fixed in 4% paraformaldehyde solution for tissue immunofluorescence analysis. In this study, 5 MARVELD1+/+ mice, 5 MARVELD1+/− mice, and 5 MARVELD1−/− mice were included. Investigators undertaking the animal monitoring were blinded to the genotype of the mice.
Sun H., Liu C., Han F., Lin X., Cao L., Liu C., Ji Q., Cui J., Yao Y., Wang B., liao Y., Nie H., Zhang Y, & Li Y. (2023). The regulation loop of MARVELD1 interacting with PARP1 in DNA damage response maintains genome stability and promotes therapy resistance of cancer cells. Cell Death and Differentiation, 30(4), 922-937.
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3

Cytotoxicity Assay of KBrO3 in U2OS Cells

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U2OS-FAP-TRF1 (WT and DDB2 KO or control and OGG1 KD) cells were plated in 6-well plates 24 h prior to treatment. The next day, cells were treated with KBrO3 (Sigma 309087), (0–20 mM) for 1 h at 37 °C. After treatment, cells were trypsinized and counted, and 800 cells were plated in 6 cm dishes for the DDB2 KO experiment, and 500 cells were plated in each well of a 6-well plate for the OGG1 KD experiment (in triplicate for each condition). Cells were then allowed to recover for 8 days. On day 8, cells were fixed with 4% formaldehyde in PBS for 15 min at room temperature and colonies were stained using a 0.1% crystal violet, 20% methanol solution for 30 min at room temperature. The plates were washed with water and dried overnight before counting.
Kumar N., Theil A.F., Roginskaya V., Ali Y., Calderon M., Watkins S.C., Barnes R.P., Opresko P.L., Pines A., Lans H., Vermeulen W, & Van Houten B. (2022). Global and transcription-coupled repair of 8-oxoG is initiated by nucleotide excision repair proteins. Nature Communications, 13, 974.
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4

Isotope-Labeled Internal Standards for Metabolomics

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VOSO4·×H2O and KBrO3 were
purchased from Sigma-Aldrich (St. Louis, MO). Nitric acid was purchased from
MilliporeSigma (Burlington, MA). V2O5 nanoparticles were
purchased from US Research Nanomaterials, Inc. (Houston, TX). An internal
standard mixture for mass spectral analysis consisted of
[13C6]-D-glucose, [15N]-indole,
[2-15N]-L-lysine dihydrochloride,
[13C5]-L-glutamic acid,
[13C7]-benzoic acid,
[3,4-13C2]-cholesterol, [15N]-L-tyrosine,
[trimethyl-13C3]-caffeine,
[15N2]-uracil, [3,3-13C2]-cystine,
[1,2-13C2]-palmitic acid, [15N ,
13C5]-L-methionine, [15N]-choline chloride,
and
2’-deoxyguanosine-15N2,13C10-5’-monophosphate,
all from Cambridge Isotope Laboratories, Inc (Andover, Pennsylvania). Ultra-pure
water (Milli-Q; Sigma-Aldrich, St. Louis, MO) was used as indicated. All
reagents were analytical grade or better unless otherwise stated.
He X., Jarrell Z.R., Ryan Smith M., Thi Ly V., Liang Y., Orr M., Go Y.M, & Jones D.P. (2022). Metabolomics of V2O5 nanoparticles and V2O5 nanofibers in human airway epithelial BEAS-2B cells. Toxicology and applied pharmacology, 459, 116327.
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5

Larval Oxidative Stress Assay

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Synchronized L1 larvae were obtained by hypochlorite treatment and were treated with indicated concentrations of KBrO3 (309087 Sigma-Aldrich) in liquid S-basal medium with OP50 for 24 h at 20 °C on a shaking platform. A minimum of 250 animals was used for each KBrO3 dose and each treatment was conducted in triplicate. Larval stages were identified using large particle flow cytometry.
Babu V, & Schumacher B. (2016). A C. elegans homolog for the UV-hypersensitivity syndrome disease gene UVSSA. DNA repair, 41, 8-15.
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6

Mineral Electrolyte Solution Preparation

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Ca(NO 3 ) 2 (99%), Mg(NO 3 ) 2 (99%), NaNO 3 (99%), CaCl 2 (99%), CaSO 4 (99%), MgCl 2 (99%), L-glutathione reduced, L-cysteine, DTNB, KBrO 3 and MOPS were purchased from Sigma-Aldrich (Steinheim, Germany); NaCl (99%) from VWR International (Leuven, Belgium) and CaCO 3 (99%) from Acros Organics (Geel, Belgium). Ultrapure water (MilliQ, minimum resistivity 18.2 MO) was used to prepare the mineral electrolyte solution series.
Lauwers K., Breynaert E., Rombouts I., Delcour J.A, & Kirschhock C.E. (2016). Water electrolyte promoted oxidation of functional thiol groups. Food chemistry, 197 Pt B.
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