We purchased MC3T3-E1 cells, a cell line derived from newborn mouse calvarial osteoblasts, from the Riken BioResource Center (Tsukuba, Japan). Mouse primary osteoblasts were isolated from calvaria of newborn ddY mice provided by Japan SLC Inc. (Hamamatsu, Japan) using a conventional method,(22 (link)) according to a protocol approved by the ethical board for animal experiments of Showa University (approval number 17055). Both MC3T3-E1 cells and mouse primary osteoblasts were cultured in αMEM (Fujifilm Wako Pure Chemical Co., Tokyo, Japan) supplemented with 10% fetal bovine serum (FBS), penicillin G, streptomycin, and amphotericin B at 37°C in a humidified atmosphere of 5% CO2/95% air. The proliferation of the cells was assessed by counting the viable cells after trypsinization and staining with trypan blue.
Mc3t3 e1 cells
Manufactured by Fujifilm
Sourced in Japan
MC3T3-E1 cells are a commonly used cell line derived from mouse calvaria. They are a well-established model for the study of osteoblast differentiation and function.
Automatically generated - may contain errors
Lab products found in correlation
α mem, by Fujifilm (1 mentions)
Mc3t3 e1 cells, by RIKEN BioResource Center (1 mentions)
Penicillin, by Fujifilm (1 mentions)
Osteoblast inducer reagent, by Takara Bio (1 mentions)
Fetal bovine serum (fbs), by Cytiva (1 mentions)
Penicillin, by Cytiva (1 mentions)
Dulbecco modified eagle medium (dmem), by Nissui Pharmaceutical (1 mentions)
2 protocols using mc3t3 e1 cells
1
Mouse Osteoblast Isolation and Culture
2
Cell Culture and Differentiation Protocols
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TSA201 cells, a clone of human embryonic kidney 293 cells (Tagami et al. 1999) (link), were grown in DMEM (Nissui Pharmaceutical, Tokyo, Japan) supplemented with 10% fetal bovine serum (FBS; HyClone laboratories Inc, Logan, UT, USA), penicillin (100 U/mL), streptomycin (100 μg/mL), and l-glutamine (2 mM). HepG2 human liver cancer cells and MC3T3-E1 mouse osteoblast-like cells were purchased from RIKEN BioResource Center (Wako City, Japan). HepG2 cells were grown in DMEM with the same supplements used for TSA201 cells. MC3T3-E1 cells were grown in MEMα (Wako Pure Chemical Industries) with 10% FBS, penicillin (100 U/mL), streptomycin (100 μg/mL), and l-glutamine (2 mM). For cell differentiation experiments, MC3T3-E1 cells were seeded in six-well plates (5.0 × 10 5 cells/well) and incubated overnight. Subsequently, the medium was changed to osteoblast differentiation medium, using the Osteoblast-Inducer Reagent (Takara Bio). All cells were incubated at 37°C in a humidified atmosphere containing 5% CO 2 .
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