Silver nitrate agno3

PVA (molecular weight (MW) ~60,000) and PEG (MW ~60,000 and ~500,000) are purchased from Sigma-Aldrich. Silver nitrate (AgNO₃) and polystylene (PS) are purchased from Wako. All the chemicals are of reagent grade and used as purchased without any further purification.

OI-iPSC constructs were fixed with 10% neutral-buffered formalin solution (Wako Pure Chemical) for 1 day. Next, the specimens were embedded in paraffin, followed by the preparation of sequential sections with 4 μm thickness using a microtome. Subsequently, the paraffin-embedded sections were evaluated using histological and immunofluorescent staining. The histological staining performed in this study was standard hematoxylin and eosin (HE) staining, methylene blue-counterstained von Kossa staining, and Movat's pentachrome staining. The paraffin-embedded sections were deparaffinized with xylene (Wako Pure Chemical) and hydrated through graded alcohol to distilled water prior to HE staining and methylene blue-counterstained von Kossa staining. For methylene blue-counterstained von Kossa staining, the specimens were incubated with 5% silver nitrate (AgNO₃; Wako Pure Chemical) under ultraviolet (UV) light for 10 minutes, then rinsed in two changes of distilled water. Next, the slides were incubated in 5% sodium thiosulfate (Sigma-Aldrich) for 5 minutes to eliminate unreacted silver and washed with running water for 5 minutes. Subsequently, nuclear counterstaining was performed using 1% methylene blue (Wako Pure Chemical) in 10 mM borate buffer. For Movat's pentachrome staining, the staining was performed as previously reported [24 (link)–26 (link)].

1-vinylimidazole and 1-bromobutane were purchased from Tokyo chemical industry Co., Ltd. (Tokyo, Japan). Lithium bis(trifluoromethanesulfonyl)imide (LiTFSI), 2,2′-azobis(isobutyronitrile), silver nitrate (AgNO₃), acetone, and methanol were obtained from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Deuterium oxide (D₂O) was purchased from Cambridge Isotope Laboratories, Inc. (Massachusetts, USA) as a solvent for NMR measurement. methanol was used after distillation. Other chemicals were used as received. DI water was obtained by using Elix system (Tokyo, Japan).

Citrate-coated Ag NPs (40 nm in diameter, Cat No. 730807) were purchased from Sigma Aldrich. The silver nitrate (AgNO₃) was purchased from FUJIFILM Wako Pure Chemical Corporation. A non-pathogenic E. coli strain (FDA strain Seattle 1946) was obtained from American Type Culture Collection (ATCC). The Luria–Bertani (LB) broth and LB agar plate for bacteria culture were purchased from TEKnova.