Serum samples were stored in a −20°C freezer. All serum sample measurements were performed in a Cobas 6000 E601 analyser (Roche Diagnostics GmbH, Mannheim, Germany) using electrochemiluminescence (ECL) technology for immunoassay analysis. Quantitative determination of antibodies (including IgG) to SARS-CoV-2 spike (S) protein receptor binding domain (RBD) was performed using the Elecsys Anti-SARS-CoV-2 S immunoassay (Roche). Antibody levels higher than 0.8 U/L were considered positive (measurement range 0.4–250 U/mL). Values higher than 250 U/mL were diluted 1:20 to obtain the exact value. 25(OH)D (vitamin D) and DHEAS levels were assessed at the 3 months post-vaccination timepoint. The measuring range for DHEAS is 0.2–1000 µg/dL and for vitamin D is 3.00–120 ng/mL, respectively.
Cobas 6000 e601 analyser
Manufactured by Roche
The Cobas 6000 E601 analyzer is a diagnostic instrument used in clinical laboratories for the automated analysis of various chemical and immunological tests. It is designed to perform a wide range of in vitro diagnostic assays, including those for clinical chemistry, immunochemistry, and specific protein analysis.
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Lab products found in correlation
2 protocols using cobas 6000 e601 analyser
1
SARS-CoV-2 Antibody and Vitamin D Assessment
2
HIV Serological and Molecular Diagnostics
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The Roche® Cobas® 6000 E601 analyser with the Roche® Elecsys® HIV combi PT fourth generation assay was used as the screening and the Abbott® ARCHITECT® HIV Ag/Ab Combo® as the confirmatory test for HIV serology. Both are 4th generation HIV serology assays, combining antibody with antigen detection. Both tests are sandwich microparticle immunoassays, where Abbott® uses chemiluminescence and Roche® uses electrochemiluminescence.
The Roche® Cobas AmpliPrep®/Cobas TaqMan® HIV-1 Qualitative Test version 2 (qualitative total nucleic acid PCR) and the Roche® Cobas AmpliPrep®/Cobas TaqMan® HIV-1 Quantitative Test version 2 (quantitative RNA PCR) were used as tie-breaker assays to determine infection status for patients with indeterminate HIV serology using a subsequent fresh new sample. These assays employ the principle of reverse transcriptase real-time polymerase chain reaction (PCR) to detect HIV nucleic acids.
The Roche® Cobas AmpliPrep®/Cobas TaqMan® HIV-1 Qualitative Test version 2 (qualitative total nucleic acid PCR) and the Roche® Cobas AmpliPrep®/Cobas TaqMan® HIV-1 Quantitative Test version 2 (quantitative RNA PCR) were used as tie-breaker assays to determine infection status for patients with indeterminate HIV serology using a subsequent fresh new sample. These assays employ the principle of reverse transcriptase real-time polymerase chain reaction (PCR) to detect HIV nucleic acids.
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