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2 protocols using hydroxytyrosol 4 β d glucoside

1

Quantification of Phenolic Compounds

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3,4-dihydroxyphenylglycol was obtained from Sigma-Aldrich (Deisenhofer, Germany). Tyrosol was obtained from Fluka (Buchs, Switzerland) and hydroxytyrosol was obtained from Extrasynthese (Lyon Nord, Geney, France). hydroxytyrosol 4-β-d-glucoside and trifluoroacetic acid (TFA) were purchased from Sigma-Aldrich (Madrid, Spain). Acetonitrile (HPLC grade) was obtained from Panreac Quimica S.A. (Barcelona, Spain). Ultrapure water was obtained from a Milli-Q water system (Millipore, Milford, MA, USA).
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2

Analysis of Phytosterols and Triacylglycerols

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-sitosterol (100 g/mL in chloroform, analytical standard) and triolein (Glyceryl trioleate, 61%) were obtained from Supelco (PA and WI, US). Chlorotrimethylsilane (TMCS) (GC Grade) was obtained from Sigma-Aldrich (Damstadt, Germany)chloroform (GPR Rectapur) and n-hexane (HPLC Grade) were obtained from VWR (Fotenay-sous-Bois, France). 5α-cholestane (99.57%, GC Grade) and pyrogallol (HPLC) were obtained from Sigma-Aldrich (Jerusalem, Israel and Dorset, UK). n-hexane (99%, GC grade) was obtained from Sigma-Aldrich (Israel). Sodium chloride (ACS grade) was obtained from Panreac (Barcelona, Spain). -sitosterol (75.8% -sitosterol, 10.3% beta-sitostanol, 7.7%
campesterol), pyridine (extra dry, 99.5%) and hexamethyldisilazane (HMDS) (98%, reagent plus) were obtained from Acros Organics (France, Geel, Belgium and Germany).
Hydrochloric acid (37%) and diethyl ether were obtained from Carlo Erba (Rodano, Italy and Val-de-Reuil, France). Potassium hydroxide (90%, flakes, pure) and ethanol (absolute) were obtained from Scharlau (Sentmenat, Spain). 5-hydroxymethylfurfural, Hydroxytyrosol 4-β-d-Glucoside, vanillic acid, p-coumaric acid and 3,4dihydroxyphenylglycol were obtained from Sigma-Aldrich (Deisenhofer, Germany).
Tyrosol was obtained from Fluka (Buchs, Switzerland). Hydroxytyrosol, oleuropein and apigenin were obtained from Extrasynthese (Lyon Nord, Geney, France).
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