The ALT test was performed for both patients and control samples to evaluate the effect of viral induced HCC on liver. The ALT kit was purchased from Merck (Darmstadt, Germany). Blood samples of both patients and control were collected in EDTA (Ethylene diamine tetraacetic acid)-vacationers’ tubes that were purchased from Becton, Dickinson and Company, USA. The manufacturer’s protocol of ALT kit was followed. The ALT concentration was checked through Microlab 300 semi-automated spectrophotometer at 340 nm.
Alt kit
Manufactured by Merck Group
Sourced in Germany, Sao Tome and Principe, Japan
The ALT kit is a laboratory diagnostic tool used to measure the levels of the enzyme alanine aminotransferase (ALT) in biological samples. ALT is an enzyme found primarily in the liver and is used as a marker for liver function. The kit provides a quantitative analysis of ALT concentrations, which can be useful in the assessment of liver health and the detection of certain liver-related disorders.
Automatically generated - may contain errors
Lab products found in correlation
Ast kit, by Merck Group (3 mentions)
Ethylene diamine tetraacetic acid (edta), by BD (1 mentions)
O dianisidine, by Merck Group (1 mentions)
Actin, by Cell Signaling Technology (1 mentions)
Ubiquitin, by Cell Signaling Technology (1 mentions)
Human serum albumin (hsa), by Baxter (1 mentions)
Traf6, by Cell Signaling Technology (1 mentions)
D galactosamine, by Merck Group (1 mentions)
Real time pcr kit, by Takara Bio (1 mentions)
Stat1, by Cell Signaling Technology (1 mentions)
Magnetic rna protein pull down kit, by Thermo Fisher Scientific (1 mentions)
P jnk, by Cell Signaling Technology (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
7 protocols using alt kit
1
Evaluating Viral-Induced Liver Damage
2
Serum Biomarkers in Mice Model
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The activities of serum endotoxin, D lactic acid and DAO were measured from the serum of 5 mice in each group. The content of endotoxin was determined by modified substrate azo dye method using kit purchased from Shanghai Biochemical Institute. Colorimetric detection was conducted using the 721 spectrophotometer (Shanghai Precision and Scientific Instrument Corporation). D lactic acid was detected by modified enzyme spectrophotometric method. D lactic acid standard solution (69806) and lactate dehydrogenase (D-LDH; 61309) were purchased from Sigma-Aldrich; Merck KGaA. DAO was determined by o-dianisidine reagent method. Cadaverine dihydrochloride (cat. no. D9143), o-dianisidine (cat. no. 540765), DAO standard solution (cat. no. 77332) and horseradish peroxidase (cat. no. MAK037) were purchased from Sigma-Aldrich; Merck KGaA. ALT in serum was determined using 96-well spectrophotometry using the ALT kit (cat. no. SEA207Mu; Shanghai Chao Yan Biotechnology Co., Ltd.). The operation was performed according to the kit protocol and the experiment was repeated 3 times.
3
Investigating Inflammatory Liver Injury Mechanisms
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Human serum albumin (HSA) was obtained from Baxter (Deerfield, IL, United States). LPS, D-galactosamine, ALT kit, and AST kit were purchased from Sigma-Aldrich (St Louis, MO, United States). IL-6, IL-22, HMGB1 ELISA kits were purchased from BIKW Co., Ltd. (Beijing, China). Antibodies against Ubiquitin (Cat.3936), TRAF6 (Cat.8028), p38 (Cat.9212), p-p38 (Cat.9216), p65 (Cat.8242), p-p65 (Cat.3033), JNK (Cat.9252), p-JNK (Cat.4668), STAT1 (Cat.14995), and Actin (Cat.3700) were obtained from Cell Signaling Technology (United States). The magnetic RNA-Protein Pull-Down Kit was purchased from Thermo Fisher (United States). Real-time PCR kits were from Takara (Japan). NEAT1 lentivirus, sh-NEAT1 lentivirus, AAV8, and AAV8-NEAT1 were purchased from Genechem (China).
4
Serum ALT and AST Quantification
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5
Serum ALT Measurement Protocol
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Serum ALT levels were detected using an ALT kit (Sigma Aldrich) according to the manufacturer’s instructions.
6
Metabolic Effects of Sphk2 Deficiency in Mice
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WT and Sphk2-KO mice on a C57BL/6 J background were used according to protocols (#2019-033) approved by Research Ethics and Governance Office, Royal Prince Alfred Hospital, Sydney, Australia. Sphk2-KO mice were obtained from Dr Richard Proia, National Institutes of Health, USA [67 (link)]. Mice were housed in a temperature-controlled, pathogen-free environment on a 12-hour light/dark cycle, and allowed food and water ad libitum. No statistical methods were used to predetermine the sample size. The sample size was estimated based on similar published studies indicating an up to 68.8% incidence of HCC tumors after HFD feeding [32 (link)–35 ]. We adopted a sample size of n = 14 per group, which was sufficient to achieve statistically significant differences. Male mice aged 8 weeks with matched body weight were fed with an HFHSD, using the recipe detailed in [68 (link)] with no cholesterol, for 46 weeks. Liver tissue and plasma were collected after 16 h starvation at the endpoint of the project. Levels of plasma NEFA, TC, TG, and ALT activity were analyzed using colorimetric assays (NEFA, TC, and TG kits from Fujifilm Wako Diagnostics, Osaka, Japan; ALT kit from Sigma-Aldrich, St. Louis, USA), as described previously [69 (link)].
7
Serum ALT and AST Measurement
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