GOx (glucose oxidase from Aspergillus niger, >100 U) and HRP (peroxidase from horseradish, >300 U) were purchased from Sigma-Aldrich (St. Louis, MO). All DNA molecules used in this study were synthesized by Sangon Biotech Co. Ltd. (Shanghai, China). The DNA sequences were listed in Table 1 . Phi29 DNA Polymerase (10 U µl−1), reaction buffer for Phi29 DNA Polymerase were purchased from Thermo Fisher Scientific (MA, United States). T4 DNA Ligase (4 U µl−1), buffer for T4 DNA Ligase with 10 mM ATP were purchased from New England Biolabs. Inc (Beijing, China). dNTP mixture (2.5 mM) was purchased from Sangon Biotech Co. Ltd. (Shanghai, China). Tris (2-carboxyethyl) phosphine (TCEP), sulfosuccinimidy l-4-(N-maleimido-methyl) cyclohexane-1-carboxylate (sulfo-SMCC), 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS), bovine serum albumin (BSA), 4′,6-diamidino-2-phenylindole (DAPI), fluorescein isothiocyanate (FITC), and rhodamine B isothiocyanate (RhoB) were purchased from Aladdin (Beijing, China). The agarose was obtained from SolaiBao Technology Co. Ltd. (Beijing, China). All the other chemicals and reagents were of analytical grade. All chemicals were used as received without further purification. The solutions were prepared using ultrapure water which was purified by a UPR series ultrapure water device.
Dntp mixture
Manufactured by Sangon
Sourced in China
DNTP mixture is a solution containing a combination of four deoxynucleotide triphosphates (dATP, dCTP, dGTP, and dTTP) commonly used in various molecular biology applications, such as DNA amplification and sequencing.
Automatically generated - may contain errors
Lab products found in correlation
T4 dna ligase, by New England Biolabs (1 mentions)
Phi29 dna polymerase, by Thermo Fisher Scientific (1 mentions)
Quikchange site directed mutagenesis kit, by Agilent Technologies (1 mentions)
Bst dna polymerase large fragment, by New England Biolabs (1 mentions)
Chitosan, by Sangon (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Rnase inhibitor, by Takara Bio (1 mentions)
Dna probe, by Sangon (1 mentions)
Generuler ultra low range dna ladder, by Thermo Fisher Scientific (1 mentions)
Tbe buffer, by Sangon (1 mentions)
Syto9, by Thermo Fisher Scientific (1 mentions)
Bst dna polymerase large fragment, by Vazyme (1 mentions)
Nt bstnbi, by New England Biolabs (1 mentions)
Scrfi, by New England Biolabs (1 mentions)
Lb agar, by Beijing Land Bridge Technology (1 mentions)
Idt oligo analyzer 3, by Integrated DNA Technologies (1 mentions)
2 2 azinobis 3 ethylbenzothiazoline 6 sulfonic acid (abts), by Sangon (1 mentions)
Lb broth, by Beijing Land Bridge Technology (1 mentions)
5 protocols using dntp mixture
1
Enzyme-Aided DNA Manipulation Protocol
2
ASFV Detection Protocol Development
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Unless otherwise stated, all the chemicals were purchased from Sigma-Aldrich (MO, USA). Capillaries were purchased from Zhong Cheng Quartz Glass (Beijing, China). Polydimethylsiloxane (PDMS) precursor was purchased from Dow Corning (MI, USA). Ultra-Ever Dry paint was purchased from UltraTech (FL, USA). Bst DNA polymerase large fragment was purchased from New England BioLabs (MA, USA). Chitosan, dNTP mixture, and agarose B were purchased from Sangon Biotech (Shanghai, China). QuikChange Site-Directed Mutagenesis Kit was purchased from Agilent Technologies (CA, USA). PCR Kit and LAMP Kit for Rapid Detection of African Swine Fever Virus were purchased from Yoyoung Biotech (Guangzhou, China). The genomic DNA samples of ASFV are provided by National African Swine Fever Regional Laboratory, South China Agricultural University (Guangzhou, China). The genomic DNA samples of other swine viruses, including dsDNA virus PRV (pseudorabies virus), ssDNA virus PCV2 (porcine circovirus type 2) and PPV (porcine parvovirus), are obtained from Huazhong Agricultural University (Wuhan, China).
3
Sensitive RNA Detection Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
T4 RNA ligase 2, phi29
DNA polymerase, and endonuclease IV (Endo IV) were purchased from
New England Biolabs (Ipswich, MA, USA). HPLC-purified miRNAs, RNase
inhibitor, and RNase-free water were purchased from Takara Biotechnology
Co., Ltd. (Dalian, China). The DNA probes, 5× TBE buffer (225
mM Tris–boric acid, 50 mM EDTA, pH 8.0), and dNTP mixture were
obtained from Sangon Biotech Co., Ltd. (Shanghai, China). GeneRuler
Ultra Low Range DNA Ladder was obtained from Thermo Fisher Scientific
Inc. (Waltham, MA, USA). RPMI 1640 medium, penicillin, 15% heat-inactivated
fetal bovine serum, and streptomycin were obtained from Thermo Scientific
HyClone (MA, USA). Sequences of DNA probes and miRNAs are given in
Table S1,Supporting Information . All other
reagents were of analytical grade and used without additional purification.
RNase-free water was used in all experiments.
DNA polymerase, and endonuclease IV (Endo IV) were purchased from
New England Biolabs (Ipswich, MA, USA). HPLC-purified miRNAs, RNase
inhibitor, and RNase-free water were purchased from Takara Biotechnology
Co., Ltd. (Dalian, China). The DNA probes, 5× TBE buffer (225
mM Tris–boric acid, 50 mM EDTA, pH 8.0), and dNTP mixture were
obtained from Sangon Biotech Co., Ltd. (Shanghai, China). GeneRuler
Ultra Low Range DNA Ladder was obtained from Thermo Fisher Scientific
Inc. (Waltham, MA, USA). RPMI 1640 medium, penicillin, 15% heat-inactivated
fetal bovine serum, and streptomycin were obtained from Thermo Scientific
HyClone (MA, USA). Sequences of DNA probes and miRNAs are given in
Table S1,
reagents were of analytical grade and used without additional purification.
RNase-free water was used in all experiments.
4
Rapid LAMP Assay for Salmonella Detection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Bst DNA polymerase (Large Fragment) was purchased from Vazyme Biotech Co., Ltd. (Nanjing, China). Nt.BstNBI, ScrFI, DraI, and agarose were obtained from New England Biolabs (Ipswich, MA, USA). Syto 9 was achieved from Thermo Fisher (Waltham, MA, USA). 4-(2-Hydroxyethyl) piperazine-1-ethanesulfonic acid (HEPES), hemin, dNTP mixture, and ABTS were all supplied by Sangon Biotech (Shanghai, China) Co., Ltd. LB agar and LB broth were offered by Beijing Land Bridge Technology Co., Ltd. (Beijing, China) 30% H2O2 was bought from Sigma-Aldrich, Inc. (Burlington, MA, USA).
The gene of invasion protein A (invA) was selected as the reference gene for amplification (GenBank accession no. NC_003197). Conventional LAMP primers were synthesized according to the previous report [32 (link)]. Inner primers incorporated with the recognition site (GAGTC) of nicking endonuclease, Nt.BstNBI, are termed as Nick-BIP and Nick-FIP. All the sequences were evaluated with IDT Oligo Analyzer 3.1 (Integrated DNA Technologies, Coralville, IA, USA). The oligonucleotides were synthesized by Sangon Biotech (Shanghai, China) Co., Ltd. Sequences used in this work are listed inTable S1 , and the corresponding template sequence is displayed in Figure S1 .
The gene of invasion protein A (invA) was selected as the reference gene for amplification (GenBank accession no. NC_003197). Conventional LAMP primers were synthesized according to the previous report [32 (link)]. Inner primers incorporated with the recognition site (GAGTC) of nicking endonuclease, Nt.BstNBI, are termed as Nick-BIP and Nick-FIP. All the sequences were evaluated with IDT Oligo Analyzer 3.1 (Integrated DNA Technologies, Coralville, IA, USA). The oligonucleotides were synthesized by Sangon Biotech (Shanghai, China) Co., Ltd. Sequences used in this work are listed in
5
DHBV Detection and Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
DHBV seeds were provided by Xuzhou Medical College (Xuzhou, China). Protease K, Taq DNA polymerase, PCR buffer, dNTP mixture and MgCl2 were the products of Sangon Biological Engineering Technology & Services Co., Ltd. (Shanghai, China). Riboflavin sodium phosphate was the product of Sigma-Aldrich (St. Louis, MO, USA). Duck hepatitis B surface antigen (DHBsAg) kit, microplate reader, and a microplate washer were provided by (Biosharp, Hefei, China). Blood was provided by the Xuzhou Blood Center (Xuzhou, China). A viral inactivation cabinet was sourced from Zibo Zhongbaokang Medical Equipment Co., Ltd. (Shandong, China). Quantitative fluorescence-polymerase chain reaction (QF-PCR) amplifier and nucleic acid protein detector were sourced from Bio-Rad Laboratories (Hercules, CA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!