All X-band CW EPR experiments were performed on a Bruker E500 EPR spectrometer (University of Florida). Low temperature CW EPR experiments were performed using a Bruker high-Q resonator (4122 SHQE) that was placed in an Oxford continuous flow cryostat (ESR900) and cooled using liquid He. A Lakeshore temperature regulator was used to maintain the temperature at 100 K. Spectra were collected with 2 mW of MW power at a center field of 3358 G, a sweep width of 300 G, and a modulation frequency of 100 kHz at an amplitude of 1 G. CW EPR experiments at 282 K, above the phase transition temperature of the lipids, were collected on a Bruker E500 EPR spectrometer equipped with a Bruker dielectric resonator (4123D). The sample temperature was maintained by flowing N2 gas through a copper heat exchanger in a water bath and then through the sample space in the resonator. Spectra were collected with 2 mW of MW power at a center field of 3475 G, a sweep width of 300 G, and a modulation frequency of 100 kHz with 0.1 G modulation amplitude. The ratio d1/d0 was determined as described in the text and used to predict distances as previously described.30 (link), 31 (link), 32 (link) All EPR spectra were baseline corrected and area normalized using software generously provided by Altenbach C. and Hubell W.L. (UCLA).
E500 epr spectrometer
Manufactured by Bruker
Sourced in China
The E500 EPR spectrometer is a laboratory instrument designed for electron paramagnetic resonance (EPR) spectroscopy. It is used to detect and analyze the presence of unpaired electrons in molecules or materials. The core function of the E500 EPR spectrometer is to measure the absorption or emission of microwave radiation by the sample under investigation, which provides information about the electronic structure and properties of the sample.
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Lab products found in correlation
4 protocols using e500 epr spectrometer
1
CW EPR Spectroscopy of Lipid Samples
2
Porphyrin-Mediated Electrolytic Hydrogen Production
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All reagents were purchased from commercial suppliers and were used as received unless otherwise noted. Acetonitrile was dried by distillation with calcium hydride. Porphyrin ligands were prepared according to the methods reported previously.25 Tetrabutylammonium hexafluorophosphate (Bu4NPF6) was recrystallized from absolute ethanol. 1H NMR spectra were acquired on a Brüker spectrometer operating at 400 MHz. UV-vis absorption spectra were measured on a Hitachi U-3310 spectrophotometer. High-resolution mass spectra (HRMS) were acquired using a Brüker MAXIS. The isotopically labelled HD gas was detected by gas chromatography–mass spectrometry using a Micromeritics AutoChem-2920. X-band continuous wave electron paramagnetic resonance (EPR) measurements were carried out on a Bruker E500 EPR spectrometer at a microwave frequency of 9.45 GHz. The EPR spectrum was recorded at 298 K. The H2 produced during the controlled potential electrolysis was determined by using an SP-6890 gas chromatograph.
3
EPR Spectroscopy of Irradiated MCT
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EPR measurements were performed using a Bruker E-500 EPR spectrometer working in the X-band with a modulation frequency of 100 kHz, scanning width of 100 G, microwave power of 20 mW, time constant of 0.04096 s, and magnetic field range of 3434–3530 G. The spent MCT was irradiated at 25 °C directly in the EPR spectrometer microwave cavity by a sunlight lamp (OSRAM, Ultra Vitalux 300 W 230 v E27, China), and the EPR spectra were recorded in situ.
4
Characterization of IseG Glycyl Radical
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Continuous wave X-band electron paramagnetic resonance (EPR) spectroscopy was used to characterize the IseG glycyl radical. A 240 μL reaction mixture containing 20 mM HEPES, pH 7.5, 0.1 M KCl, 20 μM IseG, 80 μM reconstituted MBP-IseH, 1 mM SAM, 100 μM Ti(III) citrate and 5% glycerol was incubated at RT for 10 min in the glovebox. A control sample omitting Ti(III) citrate was also prepared. All samples were loaded into EPR tubes with 4 mm o.d. and 8″ length (Wilmad Lab-Glass, 734-LPV-7), sealed with a rubber stopper, removed from the glovebox and frozen in liquid nitrogen prior to EPR analysis. The perpendicular mode X-band EPR spectra were recorded using a Bruker E500 EPR spectrometer. Data acquisition was performed with Xepr software (Bruker). The EPR spectra represent an average of 30 scans and were recorded under the following conditions: temperature, 90 K; center field, 3370 Gauss; range, 200 Gauss; microwave power, 10 μW; microwave frequency, 9.44 MHz; modulation amplitude, 0.5 mT; modulation frequency, 100 kHz; time constant, 20.48 ms; conversion time, 30 ms; scan time, 92.16 s; and receiver gain, 43 dB. The experimental spectra for the glycyl radical were modeled with Bruker Xepr spin fit to obtain g values, hyperfine coupling constants, and line widths. Double integration of the simulated spectra was used to measure spin concentration.
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