Pcdna3.1 neuropilin 1 plasmids

Manufactured by GenScript

Sourced in United States

PcDNA3.1-Neuropilin-1 plasmids are a genetic engineering tool used for the expression of the Neuropilin-1 gene. Neuropilin-1 is a cell surface receptor that plays a role in various cellular processes. These plasmids can be used in research applications to study the function and regulation of Neuropilin-1 in different cell types and contexts.

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Lab products found in correlation

Hbmec, by Neuromics (2 mentions)

2 protocols using pcdna3.1 neuropilin 1 plasmids

In Vitro BBB Modeling with hBMECs

Cited 1 time

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All reagents were purchased from Millipore-Sigma (Burlington, MA, USA), unless otherwise stated. Human brain microvascular endothelial cells (hBMECs) were obtained from Neuromics (Minneapolis, MN; catalog number: #HEC02). These cells have been proved to be the most suitable human cell line for an in vitro blood–brain barrier (BBB) model [29 (link)].
Cells were cultured in a standard humidified atmosphere (37°C) containing 5% CO₂. In some experiments, cells were transfected with pcDNA3.1-Neuropilin-1 plasmids (GenScript, Piscataway, NJ).

Mone P., Gambardella J., Wang X., Jankauskas S.S., Matarese A, & Santulli G. (2021). miR-24 targets SARS-CoV-2 co-factor Neuropilin-1 in human brain microvascular endothelial cells: Insights for COVID-19 neurological manifestations. Research Square.

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In Vitro Blood-Brain Barrier Model

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

All reagents were purchased from Millipore-Sigma (Burlington, MA, USA), unless otherwise stated. Human brain microvascular endothelial cells (hBMECs) were obtained from Neuromics (Minneapolis, MN, USA; catalog number: #HEC02). These cells have been proved to be the most suitable human cell line for an in vitro blood–brain barrier (BBB) model [29 (link)].
Cells were cultured in a standard humidified atmosphere (37 °C) containing 5% CO₂. In some experiments, cells were transfected with pcDNA3.1-Neuropilin-1 plasmids (GenScript, Piscataway, NJ, USA).

Mone P., Gambardella J., Wang X., Jankauskas S.S., Matarese A, & Santulli G. (2021). miR-24 Targets the Transmembrane Glycoprotein Neuropilin-1 in Human Brain Microvascular Endothelial Cells. Non-Coding RNA, 7(1), 9.

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