Vironostika hiv 1 microelisa

The MPS data used in the present study were derived from 134 HIV-1 near full-length genomes (NFLG) with predetermined subtype by Illumina ultra-deep sequencing technology [33 (link)]. These samples were sourced from four Brazilian states (41 from São Paulo [SP], 31 from Minas Gerais [MG], 77 from Pernambuco [PE], and 53 from Rio de Janeiro [RJ]) and were previously classified by less-sensitive (LS) or “detuned” enzyme immunoassay (Vironostika HIV-1 MicroElisa; bioMérieux, Durham, NC) or an LS chemiluminescent immunoassay (Vitros HIV-1/2 Assay; Ortho Diagnostics, Rochester, NY) as non-recent or longstanding infections being Recent Infection Testing Algorithm (RITA) reactive [22 (link)]. None of the participants had been exposed to any ARV at the time of sampling (i.e. treatment-naïve). The sequences used in this study are publicly available through the Zenodo repository browser [33 (link), 34 ]; therefore, no institutional review board (IRB) approval is required.

Previously, we had described the genetic diversity of HIV-1 using partial (n = 6) and near full-length genomes (NFLG) sequence (n = 39) of human immunodeficiency virus Type 1 provirus deep sequencing data from recently infected donors at four blood centers participating in the Retrovirus Epidemiology Donor Study (REDS-II) International Program in Brazil [23 ]. Samples were classified by less-sensitive (LS) or “detuned” enzyme immunoassay (Vironostika HIV-1 MicroElisa; bioMérieux, Durham, NC) or an LS chemiluminescent immunoassay (Vitros HIV-1/2 Assay; Ortho Diagnostics, Rochester, NY) as recently infected at the time of donation based on antibody levels consistent with recent seroconversion (infected for <170 days) as previously described [24 (link)]. None of the participants received antiviral treatment before. All study subjects provided written informed consent. The study was approved by the local ethical review committee of participating institutions as well as the REDS-II collaborating centers (Blood Systems Research Institute/University of California at San Francisco, San Francisco, CA) and data coordinating center (Westat, Inc.) in the United States.