Sh mapk1

Short hairpin RNA (sh-RNA) targeting MAPK1 (sh-MAPK1) or KCNQ1OT1 (sh-KCNQ1OT1) and negative controls (sh-NC), MAPK1 overexpression vector (pcDNA3.1/MAPK1) and the negative control (empty pcDNA3.1 vector), miR-212-3p mimics/inhibitor and the negative control (NC mimics/inhibitor) were obtained from GenePharma (Shanghai, China). Sequences of plasmids used for cell transfection are provided in Table 1. HK-2 cells were seeded in 6-well plates and grown for 24 h until the cell density reached 30–50%. Later, Lipofectamine 2000 (Thermo Fisher Scientific, USA) was used to transfect shRNAs (50 nM), mimics/inhibitors (40 nM) and pcDNA3.1 vectors (10 nM) into HK-2 cells according to the manufacturer’s protocols. After 48 hours, the efficiency of cell transfection was verified by RT-qPCR.Table 1.

Sequences of plasmids used for cell transfection

Name	Sequence (5ʹ→3ʹ)
sh-MAPK1	GGACCTCATGGAAACAGATCTTTCAAGAGAAGATCTGTTTCCATGAGGTCCTTTTTT
sh-NC	AGATGACACTATAGGTCCGACTTCAAGAGAGTCGGACCTATAGTGTCATCTTTTTTT
sh-KCNQ1OT1	GGTGTTACGACTTGTTGTATTCAAGAGATACAACAAGTCGTAACACC TTTTTT
sh-NC	GATGTGATCATTCTGGTGTTTCAAGAGAACACCAGAATGATCACATCTTTTTT
miR-212-3p mimics	UAACAGUCUCCAGUCACGGCC
NC mimics	CGCCACCUAAGUAGUGCACCU
miR-212-3p inhibitor	GGCCGUGACUGGAGACUGUUA
NC inhibitor	AGGUGCACUACUUAGGUGGCG

PcDNA3.1‐CRNDE, sh‐CRNDE, pcDNA3.1‐MAPK1, sh‐MAPK1, miR‐217 mimics, anti‐miR‐217 and negative control were provided by GenePharma (Shanghai, China). Transfection of HepG2 and Huh‐7 cells was conducted using Lipofectamine^™ 2000 (Invitrogen, Carlsbad, CA, USA). Transfected cells were cultured in 6‐well plates. After 48‐h cultivation, the cells were collected for subsequent analyses.