Stromovascular cells (SVC) and adipocytes from eWAT were fractionated, as previously described [21 (link), 26 (link)]. For gene expression analysis, dissociated adipose tissue was fractionated by magnetic cell sorting (MACS) with anti-PDGFRα-PE/anti-PE-microbeads, anti-F4/80-FITC/anti-FITC-microbeads, and anti-CD31-APC/anti-APC-microbeads (Miltenyi Biotech). For adipogenic differentiation, MACS-isolated PDGFRα was expanded in Dulbecco’s Modified Eagle’s Medium (DMEM) containing 10% FBS, and then differentiated by DMEM supplemented with a standard adipogenic cocktail for 7 days, as described previously [25 (link)]. To determine levels of adipogenic differentiation, differentiated cells were labeled with 4,4-difluoro-5-(2-thienyl)-4-bora-3a,4a-diaza-s-indacene-3-dodecanoic acid (BODIPY 558/568 C12) (Invitrogen Molecular Probes) or Oil Red O (Sigma-Aldrich). Mitochondrion-labeling in live cells was performed using red-?uorescent mitochondrion-selective probe MitoTracker Red CMXRos (Thermo Fisher, Waltham, MA, USA).
Anti pdgfrα pe anti pe microbeads
Manufactured by Miltenyi Biotec
The Anti-PDGFRα-PE/anti-PE-microbeads are a laboratory tool used for the detection and isolation of cells expressing the platelet-derived growth factor receptor alpha (PDGFRα) protein. The microbeads are conjugated with antibodies specific to PDGFRα and the fluorescent dye phycoerythrin (PE), enabling the identification and enrichment of PDGFRα-positive cells from complex samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti f4 80 fitc anti fitc microbeads, by Miltenyi Biotec (2 mentions)
Mitotracker red cmxros, by Thermo Fisher Scientific (1 mentions)
Oil red o, by Merck Group (1 mentions)
Cd44 fitc, by BioLegend (1 mentions)
Anti pdgfrα apc, by BioLegend (1 mentions)
Lsr 3, by BD (1 mentions)
F4 80 apc, by BioLegend (1 mentions)
2 protocols using anti pdgfrα pe anti pe microbeads
1
Adipocyte Fractionation and Differentiation
2
Isolation and Analysis of Adipose Stromal and Macrophage Populations
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Stromovascular cell (SVC) fractions from mouse gWAT were isolated, as previously described [6] (link). For EdU detection, fixed SVCs were processed for Click-it reaction first, followed by cell-surface marker staining. Antibodies used for flow cytometry analysis were the following: anti-PDGFRα-APC (Biolegend, cat # 135907) for ASCs, CD44-FITC (Biolegend, cat # 103021), and F4/80-APC (Biolegend, cat # 123115) for ATMs. Analytic cytometry was performed using BD LSR III (BD Biosciences) flow cytometers. Raw data were processed using FlowJo software (Tree Star). For gene expression analyses by qPCR or RNAseq, ATMs and ASCs were isolated by magnetic cell sorting (MACS) with anti-F4/80-FITC/anti-FITC-microbeads and anti-PDGFRα -PE/anti-PE-microbeads, respectively (Miltenyi Biotech).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!