Perforin fitc

Manufactured by Mabtech

Perforin-FITC is a fluorescently labeled antibody that binds to the cytolytic protein perforin. Perforin is a key component of the cytotoxic granules released by cytotoxic T cells and natural killer cells to induce target cell lysis.

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Lab products found in correlation

Live dead fixable aqua dead cell stain, by Thermo Fisher Scientific (2 mentions) Qdot 655, by Thermo Fisher Scientific (1 mentions) Ccr7 fitc, by R&D Systems (1 mentions) Alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Cd69 ecd, by Beckman Coulter (1 mentions) Cd3 percp cy5, by Beckman Coulter (1 mentions) Cd4 fitc, by Beckman Coulter (1 mentions) Cd4 percp cy5, by Beckman Coulter (1 mentions) Ccr7 percp cy5, by Thermo Fisher Scientific (1 mentions) Cd3 pe cy7, by Beckman Coulter (1 mentions) Cd95 apc, by Thermo Fisher Scientific (1 mentions) Cd28 pe cy7, by Thermo Fisher Scientific (1 mentions) Mip 1β pe, by Beckman Coulter (1 mentions) Cd3 apc cy7, by BD (1 mentions) Cd4 pe cy5, by Thermo Fisher Scientific (1 mentions) Cd28 ecd, by Beckman Coulter (1 mentions) Ki67 fitc, by BD (1 mentions) Cd8 bv570, by BioLegend (1 mentions) Cd20 bv650, by BioLegend (1 mentions) Cd69 apc, by BD (1 mentions)

2 protocols using perforin fitc

Multiparameter Flow Cytometry Assay

Cited 1 time

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Conjugated antibodies and staining reagents included MIP-1β-PE, CD3-PB, CD3-PE-Cy7, CD3-PerCP-Cy5.5, CD3-APC-Cy7, CD3-Horizon V450, CD4-PerCP-Cy5.5, CD4-AmCyan, CD4-FITC, CD8α-APC, CD8α-APC-Cy7, CD8α-AlexaFluor700, CD8α-FITC, CD8α-APC-H7, CD69- ECD (Beckman Coulter), CD20-Horizon V450, CCR7-FITC (R&D Systems), CD95-APC, CCR7-PerCP-Cy5.5, CD28-PE-Cy7 (eBioscience), granzyme B-AlexaFluor700, perforin-FITC (MabTech), IFNγ-PE-Cy7, TNFα- AlexaFluor700, IL-2-APC, CD95-PE, CD95-APC, and Aqua LIVE/DEAD Fixable Dead Cell Stain (Invitrogen). All reagents are from BD Biosciences unless indicated otherwise. For construction of monomers and tetramers, the following peptides were synthesized and purified to >95% by HPLC by New England Peptide LLC: p11C (CTPYDINQM), p54AS (TVPWPNASL), p54E660 (TVPWPNETL), p68A (STPPLVRLV), and TL8 (TTPESANL). The monomers and tetramers were prepared as previously described [85] (link), [86] (link). Tetramers were prepared using either streptavidin-PE (Prozyme), -APC (Prozyme), -AlexaFluor488 (Invitrogen), or -Qdot655 (Invitrogen). Monomers used in surface plasmon resonance studies were further quantified using an RC DC protein kit (Bio-Rad).

Osuna C.E., Gonzalez A.M., Chang H.H., Hung A.S., Ehlinger E., Anasti K., Alam S.M, & Letvin N.L. (2014). TCR Affinity Associated with Functional Differences between Dominant and Subdominant SIV Epitope-Specific CD8+ T Cells in Mamu-A*01 + Rhesus Monkeys. PLoS Pathogens, 10(4), e1004069.

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Comprehensive Lymphocyte Immunophenotyping

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The following antibodies were used for phenotyping and functional evaluation of lymphocytes in this study: CD107a-BV421 (Clone H4A3, Biolegend), CD4-PECy5.5 (Clone S3.5, Invitrogen), CD8-BV570 (Clone RPA-T8, Biolegend), CD14-BV650 (Clone M5E2, Biolegend), CD20-BV650 (Clone 2H7, Biolegend), CD16-BV650 (Clone 3G8, Biolegend), CD28-ECD (Clone CD28.2, Beckman Coulter), CD95-PECy5 (Clone Dx2, BD Biosciences), CD38-PE (Clone OKT10, NIH NHP Reagent Resource, University of Massachusetts, Boston, MA), CCR7- BV711 (Clone G043H7, Biolegend). CD3-APCCy7 (Clone SP34-2, BD Biosciences), granzyme B-AF700 (Clone GB11, BD Biosciences), T-bet-PECy7 (Clone 4B10, eBioscience), Ki67-FITC (Clone B56, BD Bioscience) or Ki67-BV786 (Clone B56, BD Bioscience), CD69-APC (Clone FN50, BD Bioscience) or CD69-BV605 (Clone FN50, Biolegend), Perforin-FITC (Clone pf344, MabTech), TNF-BV605 (Clone MAb11, Biolegend), IFNg-BV785 (Clone 4S.B3, Biolegend), Live Dead Fixable Aqua Dead Cell Stain (Molecular Probes).

Roberts E.R., Carnathan D.G., Li H., Shaw G.M., Silvestri G, & Betts M.R. (2016). Collapse of Cytolytic Potential in SIV-Specific CD8+ T Cells Following Acute SIV Infection in Rhesus Macaques. PLoS Pathogens, 12(12), e1006135.

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