Norepinephrine (NE) and HMGB1 were measured in plasma samples (n =8/group) using a norepinephrine ELISA kit (Abnova, Taiwan) and a HMGB1 ELISA kit (MyBioSource, CA, USA), respectively as per the manufacturer’s guidelines.
Hmgb1 elisa kit
Manufactured by MyBioSource
Sourced in United States
The HMGB1 ELISA kit is a laboratory tool used to detect and quantify the levels of the HMGB1 protein in biological samples. It employs the enzyme-linked immunosorbent assay (ELISA) technique to measure the concentration of HMGB1 present in the sample.
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5 protocols using hmgb1 elisa kit
1
Plasma Biomarkers of NE and HMGB1
2
HMGB1 Levels in Intraluminal Fluid
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Intraluminal fluid samples were collected at the end of the experiment and immediately centrifuged to remove cells and stored at −80°C until use. The levels of HMGB1 in the intraluminal fluid were assessed using a HMGB1 ELISA kit (MBS2701751, MyBioSource) following the manufacturer's instructions.
3
Quantifying HMGB1 in Biological Fluids
4
Telmisartan Attenuates Cardiac Injury
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Male Wistar albino rats (200–250 g) were employed for this study and were kept in the animal house of the department with standard laboratory conditions i.e., under controlled temperature, humidity, chow diet and the normal cycle of 12 h light and 12 h dark. All the experiments were also performed per the Animal Ethics Committee of the Second Affiliated Hospital of Dalian Medical University-wide Ethic number: 2019211. Telmisartan (Sigma, St. Louis, MO, USA) and AR-A014418 (Cayman Chemical Co., Ann Arbor, MI, USA) were dissolved in normal saline containing 10% DMSO. IL-6, IL-10, creative kinase-MB (CK-MB) and cardiac troponin I (cTnI) ELISA detection kits were purchased from Beijing Cheng Lin Biotechnology Co., Ltd., Beijing, China. HMGB1 ELISA kit was purchased from MyBioSource, Inc., San Diego, CA, USA for the quantitative determination of HMGB1 in the tissue homogenate. GSK-3β ELISA kit was purchased from Ray Biotech Pvt. Ltd., Norcross, GA, USA for estimation of total GSK-3β and p-GSK3β-S9 levels.
5
Serum HMGB1 levels in rats
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For ELISAs, rat serum was isolated from angular vein blood before and 7 days after surgery. For this purpose, the blood samples were allowed to clot for 2 hours at room temperature, and the serum was removed and stored at -80°C for later use. A rat high mobility group box 1 protein (HMGB1) ELISA kit (Catalogue Number: MBS729203, MyBioSource, USA) was used to measure the HMGB1 protein levels in the rat sera according to the manufacturer's instructions.
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