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Roti quant bradford reagent

Manufactured by Carl Roth
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Roti®-Quant Bradford reagent is a protein quantification solution used for the determination of protein concentrations in samples. It is based on the Bradford method, a widely-used colorimetric assay for protein analysis.

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Lab products found in correlation

1 mm quartz cuvette, by Hellma (1 mentions)

Close spelling variants of this product

Roti-Quant (70 citations) Roti-Quant reagent (10 citations) Bradford reagent (9 citations)

2 protocols using roti quant bradford reagent

1

Protein Quantification of Antibiotic-BSA Conjugates

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Protein concentration of each antibiotic-BSA conjugate was determined after final sterile filtration with Bradford protein assay using the Roti®-Quant Bradford reagent (Carl Roth) against BSA as reference. The assay was performed according to the manufacturer’s instructions. Briefly, a serial dilution of samples was prepared in a 96-well plate and 200 µL of Bradford reagent was added. After an incubation of 5 min at room temperature, the absorbance was measured at a wavelength of 595 nm.
Pietschmann J., Dittmann D., Spiegel H., Krause H.J, & Schröper F. (2020). A Novel Method for Antibiotic Detection in Milk Based on Competitive Magnetic Immunodetection. Foods, 9(12), 1773.
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2

Protein Sample Analysis by Bradford Assay

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Sample concentration was measured by Bradford assay against a BSA standard (Rotiquant Bradford reagent, Roth) and adjusted to 20 μM. The CD spectroscopy measurements were performed at room temperature in a ultraviolet-spectropolarimeter J750 (Jasco) using 1 mm quartz cuvettes (Hellma).
Gumiero A., Conz C., Gesé G.V., Zhang Y., Weyer F.A., Lapouge K., Kappes J., von Plehwe U., Schermann G., Fitzke E., Wölfle T., Fischer T., Rospert S, & Sinning I. (2016). Interaction of the cotranslational Hsp70 Ssb with ribosomal proteins and rRNA depends on its lid domain. Nature Communications, 7, 13563.
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