Naringenin (Tokyo Chemical Industry, Tokyo, Japan) was dissolved in dimethyl sulfoxide (DMSO; Fujifilm Wako Pure Chemical Corporation) at a concentration of 100 mg/mL. A corresponding concentration of DMSO was used for the control group.
Naringenin
Manufactured by Tokyo Chemical Industry
Sourced in Japan
Naringenin is a chemical compound that belongs to the flavonoid class. It is a naturally occurring compound found in various citrus fruits, such as grapefruit. Naringenin functions as a crystalline solid and is soluble in organic solvents.
Automatically generated - may contain errors
Lab products found in correlation
Naringin, by Tokyo Chemical Industry (2 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (2 mentions)
Dimethyl sulfoxide (dmso), by Fujifilm (1 mentions)
Peptone, by Merck Group (1 mentions)
Myricetin, by Fujifilm (1 mentions)
Quercetin, by Fujifilm (1 mentions)
Kaempferol, by Fujifilm (1 mentions)
Galangin, by Fujifilm (1 mentions)
Baicalein, by Fujifilm (1 mentions)
Chrysin, by Fujifilm (1 mentions)
Yeast extract, by Merck Group (1 mentions)
1 deoxy 1 morpholino fructose dmf, by Merck Group (1 mentions)
Nitrotetrazolium blue chloride nbt, by Sinopharm (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Kanamycin sulfate, by Fujifilm (1 mentions)
Methanol, by Fujifilm (1 mentions)
E coli bl21 de3, by Nippon Gene (1 mentions)
Ampicillin sodium, by Fujifilm (1 mentions)
Escherichia coli jm109, by Takara Bio (1 mentions)
Mighty ta cloning reagent set for primestar, by Takara Bio (1 mentions)
9 protocols using naringenin
1
Naringenin Dissolution in DMSO
2
Polyphenol Compound Procurement for Biochemical Assays
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Flavone, 6-hydroxy flavone, 3-hydroxy flavone, 7-hydroxy flavone, 6-methoxy-flavone, 6-methoxy flavanone, naringenin and naringin were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Biochanin A, genistein, apigenin, puerarin, wogonin, catechin (C), epicatechin (EC), luteolin, daidzein, daidzin, resveratrol, piceid and neohesperidin-dihydrochalcone were purchased from Aladin Co. Ltd. (Shanghai, China). Galangin, kaempferol, quercetin, myricetin, baicalein and chrysin were purchased from Wako Pure Chemical Industries (Osaka, Japan). All other polyphenols were obtained commercially from Shanghai Tauto Biotech CO., Ltd. (Shanghai, China). Peptone, yeast extract, agar, 1-deoxy-1-morpholino fructose (DMF) and bovine serum albumin (BSA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Nitrotetrazolium Blue chloride (NBT) was obtained from Sinopharm Chemical Reagent Co., Ltd. (Beijing, China). Kanamycin and carbenicillin were purchased from Shanghai Xinya Pharmaceutical Co., Ltd. (Shanghai, China). Fetal bovine serum (BP) of no phage and low endotoxin was from Sijiqing Biological Engineering Materials Co., Ltd. (Hangzhou, China). All other reagents and solvents were analytical grade and used without further purification.
3
Anti-PD-L1 Antibody and Compound Assay
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Anti‐programmed death‐ligand 1 (PD‐L1) antibody and IgG controls were purchased from BioLegend. Diphtheria toxin was obtained from FUJIFILM Wako Pure Chemical Corporation. Naringin and naringenin (Tokyo Chemical Industry) were dissolved in dimethyl sulfoxide at a concentration of 100 mM.
4
Bioactive Compounds from A. sieboldiana Seeds
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A. sieboldiana seeds from the Forestry and Forest Products Research Institute were used. Benlate wettable powder was purchased from Sumitomo Chemical Garden Products. Ampicillin sodium, kanamycin sulfate, EtOAc, isopropyl-1-thio-β-d -galactopyranoside (IPTG), and methanol (MeOH) were purchased from FUJIFILM Wako Pure Chemical. Streptomycin sulfate, p-coumaric acid (1 ), ferulic acid (2 ), caffeic acid (3 ), sinapinic acid (4 ), dihydro-p-coumaric acid (5 ), and naringenin were purchased from the Tokyo Chemical Industry. trans-Cinnamic acid (6 ) was purchased from Sigma-Aldrich. PrimeSTAR® Max DNA Polymerase, Escherichia coli JM109, restriction enzymes (NdeI, BamHI, BglII) and T-Vector pMD19 were purchased from Takara Bio. A-overhang mixture used was included in the Mighty TA-cloning Reagent Set for PrimeSTAR® from Takara Bio. pET23a(+) DNA and pETDuet-1 DNA were purchased from Novagen. The restriction enzyme NcoI and E. coli BL21(DE3) were purchased from NIPPON GENE. HPLC-grade acetonitrile (CH3CN) was purchased from Kanto Chemical. LCMS-grade CH3CN was purchased from Supelco.
5
Lipid Nanoparticle Formulation and Optimization
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Naringenin and hesperetin were purchased from Tokyo Chemical Industry (Tokyo, Japan). Polyoxyethylene sorbitan monooleate (Tween 80) and propylene glycol (PG) was from J. T. Baker (Phillipsburg, USA). Epikuron-200 (containing more than 92% of phosphatidylcholine and others of lysoPC, phosphatidic acids, and triglycerides.) was acquired from Cargill, Inc. (Minnetonka, Minnesota, U.S.). Cholesterol and paraformaldehyde were purchased from Sigma-Aldrich (St. Louis, Missouri, USA). All other chemicals and solvents were of analytical reagent grade.
6
Antioxidant Compounds and Autophagy
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Epigallocatechin, myricetin, fisetin, quercetin, kaempferol, galangin, naringenin, and Epigallocatechin gallate were purchased from the Tokyo Chemical Industry Co. (Tokyo, Japan). Lipofectamine 3000, CellROX Orange, and CellROX Deep Red were purchased from Thermo Fisher Scientific (Waltham, MA, USA), and the autophagy detection reagent, DALGreen, was purchased from Dojindo Laboratories (Kumamoto, Japan).
7
Natural Compound Sources for Research
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Potential antibiotics, trans-cinnamic acid, naringenin, acetovanillone, (+)-catechin, catechol, scopoletin, and coumarin were obtained from Tokyo Chemical Industry Co. Ltd. (Tokyo, Japan), phloretin, chlorogenic acid, and p-coumaric acid were obtained from Sigma-Aldrich (St. Louis, MO, USA), and acetosyringone was obtained from Cayman Chemical (Ann Arbor, MI, USA). Phytoalexins, capsidiol, debneyol, and rishitin were purified in the plant pathology laboratory of Nagoya University.
8
Engineered E. coli Phenylpropanoid Production
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Eriodictyol, dihydrokaempferol (DHK), and DHQ were purchased from Merck (Sigma Aldrich). p-Coumaric acid and naringenin were purchased from Tokyo Chemical Industry. TT7 (codon optimized for E. coli) was synthesized as gBlocks Gene Fragment from Integrated DNA Technologies Inc. The ATR2 gene (codon optimized for E. coli) was synthesized from GenScript. All strains and plasmids used in this study are listed in Table 1 and2, respectively. E. coli DH5α (Invitrogen) was used for routine gene cloning works. BTY5 (Kim et al., 2018) was used as a host strain for the production of all phenylpropanoids.
9
Comprehensive Phytochemical Analysis Protocol
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MCI gel CHP20P (particle diameter: 75-150 µm) was purchased from Mitsubishi Chemical (Tokyo, Japan), Sephadex LH-20 from GE Healthcare (Chicago, IL, USA), Chromatorex ODS (30-50 µm) from Fuji Silysia Chemical (Kasugai, Japan), and silica gel 60 (0.040-0.063 mm) from Merck (Kenilworth, NJ, USA). Protein-R HPLC column, arbutin, and L-tyrosine were purchased from Nacalai Tesuque (Kyoto, Japan).
Dacarpo DX-C18 HPLC column was purchased from Imtakt (Kyoto). Human leukocyte elastase and mushroom tyrosinase were from Sigma Aldrich (St. Louis, MO, USA), naringenin and 1,1-diphenyl-2-picrylhydrazyl (DPPH) from Tokyo Chemical Industry (Tokyo), and Suc(OMe)-Ala-Ala-Pro-Val-MCA from PEPTIDE INSTITUTE (Osaka, Japan). 2-(N-morpholino)-ethanesulfonic acid (MES) and 2-[4-(2-Hydroxyethyl)-1piperazinyl]-ethanesulfonic acid (HEPES) were from Dojindo Chemical Research (Kumamoto, Japan). CD3OD, pyridine-d5, and DMSO-d6 were from Cambridge Isotopic Laboratories (Andover, MA). Apigenin, 5, 7, , and other chemicals were purchased from Fujifilm Wako Pure Chemical Corporation (Osaka).
Dacarpo DX-C18 HPLC column was purchased from Imtakt (Kyoto). Human leukocyte elastase and mushroom tyrosinase were from Sigma Aldrich (St. Louis, MO, USA), naringenin and 1,1-diphenyl-2-picrylhydrazyl (DPPH) from Tokyo Chemical Industry (Tokyo), and Suc(OMe)-Ala-Ala-Pro-Val-MCA from PEPTIDE INSTITUTE (Osaka, Japan). 2-(N-morpholino)-ethanesulfonic acid (MES) and 2-[4-(2-Hydroxyethyl)-1piperazinyl]-ethanesulfonic acid (HEPES) were from Dojindo Chemical Research (Kumamoto, Japan). CD3OD, pyridine-d5, and DMSO-d6 were from Cambridge Isotopic Laboratories (Andover, MA). Apigenin, 5, 7, , and other chemicals were purchased from Fujifilm Wako Pure Chemical Corporation (Osaka).
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