Log In Sign up
The largest database of trusted experimental protocols

Epr4004

Manufactured by Abcam
Visit Supplier
Sourced in United Kingdom

EPR4004 is a monoclonal antibody that recognizes the FAS receptor, also known as CD95 or TNFRSF6. The FAS receptor is a type I transmembrane protein that plays a key role in the regulation of programmed cell death or apoptosis. EPR4004 can be used in various applications, such as flow cytometry, immunohistochemistry, and immunofluorescence, to detect and study the FAS receptor and its expression in biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Anti pakt s473, by Cell Signaling Technology (1 mentions) Anti igf2bp2, by Proteintech (1 mentions) Anti akt c67e7, by Cell Signaling Technology (1 mentions) Anti igf2bp3, by Proteintech (1 mentions) Anti pi3k p110, by Abcam (1 mentions) Anti igf2bp1 22803 1 ap, by Proteintech (1 mentions) Anti wtap, by Proteintech (1 mentions) Anti gapdh 10494 1 ap, by Proteintech (1 mentions)

2 protocols using epr4004

1

Immunostaining of Egr2 and NPAS4

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Tissue immunostaining was carried out as previously described [75 (link)]. The following primary antibodies were used: Rb-Egr2 (EPR4004, AbCam), and Rb anti-NPAS4 (orb256724, Biorbyt, Cambridge, UK). Specific secondary antibodies for each condition were used (A21429-Alexa Fluor 555 Rb-IgG, ThermoFisher Scientific), counterstained with DAPI (blue). Pictures were obtained using a Zeiss or a Nikon eclipse Ti2 microscope, with magnification as indicated and processed using ImageJ Fiji version 1.50. Densitometric analysis was obtained as previously described [75 (link)].
Petazzi P., Jorge-Torres O.C., Gomez A., Scognamiglio I., Serra-Musach J., Merkel A., Grases D., Xiol C., O’Callaghan M., Armstrong J., Esteller M, & Guil S. (2023). Global Impairment of Immediate-Early Genes Expression in Rett Syndrome Models and Patients Linked to Myelination Defects. International Journal of Molecular Sciences, 24(2), 1453.
+ Open protocol
+ Expand
2

Comprehensive Western Blot Analysis

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blot assay was conducted as previously described [24 (link)]. Antibodies used in this study were as follows: anti-GAPDH (10494-1-AP, Proteintech), anti-AKT (C67E7, Cell Signaling Technology), anti-pAKT (S473) (193H12, Cell Signaling Technology), anti-PI3K (Y388, Abcam), anti-PI3K (p110) (Y384, Abcam), anti-S1PR3 (EPR454, Abcam), anti-EGR2 (EPR4004, Abcam), anti-IGF2BP1 (22803-1-AP, Proteintech), anti-IGF2BP2 (11601-1-AP, Proteintech), anti-IGF2BP3 (14642-1-AP, Proteintech), anti-WTAP (10200-1-AP, Proteintech).
Ying Y., Ma X., Fang J., Chen S., Wang W., Li J., Xie H., Wu J., Xie B., Liu B., Wang X., Zheng X, & Xie L. (2021). EGR2-mediated regulation of m6A reader IGF2BP proteins drive RCC tumorigenesis and metastasis via enhancing S1PR3 mRNA stabilization. Cell Death & Disease, 12(8), 750.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!