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Anti progerin

Manufactured by Santa Cruz Biotechnology
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Anti-Progerin is a research-grade antibody product designed to detect and quantify the progerin protein. Progerin is a mutant form of the lamin A protein associated with the accelerated aging disorder Hutchinson-Gilford progeria syndrome. This antibody can be used in various analytical techniques, such as Western blotting, immunohistochemistry, and ELISA, to study the expression and localization of progerin in biological samples.

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Lab products found in correlation

Anti lamin a c, by Santa Cruz Biotechnology (3 mentions) Sc 81611, by Santa Cruz Biotechnology (2 mentions) Anti p16, by BD (2 mentions) Anti β tubulin, by Santa Cruz Biotechnology (2 mentions) Anti wrn, by Santa Cruz Biotechnology (2 mentions) Anti lap2β, by BD (2 mentions) Anti p21, by Cell Signaling Technology (2 mentions) Anti actin, by Santa Cruz Biotechnology (2 mentions) Anti lamin a c, by Cell Signaling Technology (2 mentions) Anti sirt1, by Abcam (2 mentions) Anti vwf, by Santa Cruz Biotechnology (2 mentions) Anti lamin b1, by Proteintech (2 mentions) Anti histone h3, by Proteintech (2 mentions) Anti gapdh, by Proteintech (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) A11001, by Thermo Fisher Scientific (1 mentions) A11012, by Thermo Fisher Scientific (1 mentions) Apotome 2, by Zeiss (1 mentions) Sc 20681, by Santa Cruz Biotechnology (1 mentions) Fluorsave reagent, by Merck Group (1 mentions)

Close spelling variants of this product

Mouse anti-progerin (2 citations)

6 protocols using anti progerin

1

Progerin and Lamin A/C Immunofluorescence

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Fibroblasts were seeded into 4 chamber-well slides (SPL Lifesciences, Korea). Cells were fixed for 15 minutes (RT) in a 4% paraformaldehyde +2% sucrose solution and then permeabilized for 3 minutes at RT using permeabilization buffer (0.5% Triton X-100, 50 mM NaCl, 300 mM sucrose, 20 mM HEPES pH 7.5, 3 mM MgCl2). Cells were then incubated with primary antibodies for 40 minutes at 37 °C (anti-progerin (1/50, sc-81611) and anti-lamin A/C (1/50, sc-20681), Santa Cruz Biotechnology). After washing, cells were incubated for 20 minutes at 37 °C with secondary antibodies (A11001, A11012, 1/400 Life Technologies). Nuclei were stained with DAPI for 10 minutes at room temperature (0.1 μg/mL, Thermofisher). Slides were mounted using FluorSave™ reagent (Merck Millipore) and observed on a fluorescence microscope (ApoTome.2 Zeiss).
Frankel D., Delecourt V., Novoa-del-Toro E.M., Robin J.D., Airault C., Bartoli C., Carabalona A., Perrin S., Mazaleyrat K., De Sandre-Giovannoli A., Magdinier F., Baudot A., Lévy N., Kaspi E, & Roll P. (2022). miR-376a-3p and miR-376b-3p overexpression in Hutchinson-Gilford progeria fibroblasts inhibits cell proliferation and induces premature senescence. iScience, 25(2), 103757.
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2

Comprehensive Antibody Panel for Cell Characterization

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Primary antibodies for FACS were anti-CD73-PE (550741, 1:100), anti-CD90-FITC (555595, 1:200) from Biosciences and anti-CD105-APC (17-1057, 1:100) from eBioscience. Primary antibodies for Western blot were anti-WRN (sc-5629, 1:500), anti-β-Actin (sc-130301, 1:3,000), anti-β-Tubulin (sc-5274, 1:3,000) from Santa Cruz Biotechnology, anti-P21 (2947, 1:2,000), anti-HP1γ (2619, 1:1,000) from Cell Signaling Technology, anti-LAP2β (611000, 1:2,000) and anti-P16 (4828, 1:200) from BD Bioscience. Antibodies for immunofluorescent staining were anti-hSMA (ZM-0003) from ZSGB-Bio, anti-Progerin (sc-81611, 1:50), anti-Lamin A/C (sc-7293, 1:200) from Santa Cruz Biotechnology, anti-HP1γ (2619, 1:500) from Cell Signaling Technology, anti-53BP1 (A300-273A, 1:500) from Bethyl Laboratories, anti-γ-H2AX (05-636, 1:500) from Millipore, anti-LAP2β (611000, 1:500), anti-hCD31 (555445, 1:200) from BD Bioscience, and anti-Ki67 (VP-RM04, 1:1,000) from Vector.
Geng L., Liu Z., Zhang W., Li W., Wu Z., Wang W., Ren R., Su Y., Wang P., Sun L., Ju Z., Chan P., Song M., Qu J, & Liu G.H. (2018). Chemical screen identifies a geroprotective role of quercetin in premature aging. Protein & Cell, 10(6), 417-435.
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3

Antibody Panel for Cellular Analysis

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Antibodies used for experiments included anti-GFP (1:1000; sc-9996; Santa Cruz Biotechnology, Dallas, TX, USA); anti-GST (1:5000; sc-138; Santa Cruz Biotechnology), anti-His (1:1000; 66005-1-lg; Proteintech, Rosemont, IL, USA), anti-Actin (1:10000; sc-47778; Santa Cruz Biotechnology), anti-Lamin A/C (1:10000; sc-376248; Santa Cruz Biotechnology), anti-Progerin (1:100; sc-81611; Santa Cruz Biotechnology), anti-Progerin (1:300; ab66587; Abcam, Cambridge, UK), anti-Ki67 (1:200; Ab15580; Abcam), anti-cyclin B1 (1:100; sc-594; Santa Cruz Biotechnology), and anti-p16-INK4A (1:500; 10883-1-AP; Proteintech).
Kang S.M., Yoon M.H., Ahn J., Kim J.E., Kim S.Y., Kang S.Y., Joo J., Park S., Cho J.H., Woo T.G., Oh A.Y., Chung K.J., An S.Y., Hwang T.S., Lee S.Y., Kim J.S., Ha N.C., Song G.Y, & Park B.J. (2021). Progerinin, an optimized progerin-lamin A binding inhibitor, ameliorates premature senescence phenotypes of Hutchinson-Gilford progeria syndrome. Communications Biology, 4, 5.
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4

Detailed Procedures for Cellular Senescence Assessment

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The reagents included carbon tetrachloride (Sigma‐Aldrich, 56‐23‐5), 30% H2O2 (Hydrogen peroxide 30%, Sigma‐Aldrich, 1.07298), selisistat (EX‐527, MedChemExpress, 49843‐98‐3), resveratrol (SRT501, MedChemExpress, 501‐36‐0), DAPI (Sigma‐Aldrich, D9542), Alexa Fluor™ 647 Phalloidin (Thermo, A22287), protease cocktails inhibitor (Beyotime, P1005) and PMSF (Phenylmethanesulphonyl fluoride, Beyotime, ST506).
The primary antibodies included anti‐α‐SMA (Boster, BM0002), anti‐vWF (Santa Cruz, SC‐365712), anti‐CD32b (ZEN‐bioscience, 382560), anti‐CD31 (PECAM‐1, Santa Cruz, sc‐18916), anti‐CD31 (Abcam, ab33858), anti‐NOX2 (Proteintech, 19013‐1‐AP), anti‐NOX4 (Proteintech, 14347‐1‐AP), anti‐Lamin A/C (Cell Signaling Technology, 4777S), anti‐Lamin B1 (Proteintech, 66095‐1‐Ig), anti‐progerin (Santa Cruz, sc‐81611), anti‐p53 (Abcam, ab131442), anti‐p53 (acetyl K381; Abcam, ab61241), anti‐SIRT1 (Abcam, ab110304), anti‐Histone H3 (Proteintech, 17168‐1‐AP) and anti‐GAPDH (Proteintech, 60004‐1). HRP‐conjugated Affinipure Goat Anti‐Mouse IgG (H + L; Proteintech, SA00001‐1), HRP‐conjugated Affinipure Goat Anti‐Rabbit IgG (H + L; Proteintech, SA00001‐2), FITC‐labelled goat anti‐rabbit IgG (H + L; Beyotime, a0562) and Cy3‐labelled goat anti‐mouse IgG (H + L; Beyotime, a0521) were used for secondary antibodies.
Luo X., Bai Y., He S., Sun S., Jiang X., Yang Z., Lu D., Wei P., Liang Y., Peng C., Wang Y., Sheng R., Han S., Li X, & Zhang B. (2021). Sirtuin 1 ameliorates defenestration in hepatic sinusoidal endothelial cells during liver fibrosis via inhibiting stress‐induced premature senescence. Cell Proliferation, 54(3), e12991.
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5

Cellular Senescence and Oxidative Stress Modulation

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The primary antibodies included anti-vWF (Santa Cruz, #sc-365712), anti-COL 1A1 (Proteintech, #14695-1-AP), anti-progerin (Santa Cruz, #sc-81611), anti-Lamin A/C (Cell Signaling Technology, #4777S), anti-Lamin B1 (Proteintech, #66095-1-Ig), anti-SIRT1 (Abcam, #ab110304), anti-LC3B (Abcam, #ab48394), anti-acetyl Lysine (Abcam, #ab22550), anti-Caveolin-1 (Cav-1) (Abcam, #ab32577), anti-Histone H3 (Proteintech, #17168-1-AP), and anti-GAPDH (Proteintech, #60004-1). The secondary antibodies included Cy3-labeled goat anti-mouse IgG (H+L) (Beyotime, #a0521), FITC-labeled goat anti-rabbit IgG (H+L) (Beyotime, #a0562), HRP-conjugated Affinipure Goat Anti-Mouse IgG(H+L) (Proteintech, #SA00001-1), and HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H+L) (Proteintech, #SA00001-2).
The reagents used included carbon tetrachloride (CCl4) (Sigma-Aldrich, #56-23-5), hydrogen peroxide (H2O2) (Sigma-Aldrich, #1.07298), N-Acetylcysteine (NAC) (MedChemExpress, #616-91-1), mitochondria 2,2,6,6-tetramethylpiperidinooxy (mito-TEMPO) (MedChemExpress, #1334850-99-5), 3-Methyladenine (3-MA) (Sigma-Aldrich, #S2767), rapamycin (sirolimus) (Sigma-Aldrich, #S1039), MCDB131 (Gibco, #10372019), 1640 (Gibco, #11875101), and fetal bovine serum (FBS) (Biological Industries, #04-007-1A).
Bai Y., Liu J., Jiang X., Li X., Zhang B, & Luo X. (2022). Nucleophagic Degradation of Progerin Ameliorates Defenestration in Liver Sinusoidal Endothelium Due to SIRT1-Mediated Deacetylation of Nuclear LC3. Cells, 11(23), 3918.
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6

Western Blot Analysis of Cellular Proteins

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1 × 106 cells were lysed in 100 μL RIPA buffer [50 mmol/L Tris-HCl (pH = 7.5), 150 mmol/L NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS] supplemented with NaF, NaVO4 and a protease-inhibitor mixture (Roche). Typically 20 μg of proteins were separated by SDS-PAGE, transferred to a PVDF membrane (Millipore), and blotted with one of the following primary antibodies and then HRP-conjugated secondary antibodies. The quantification of western blot was performed with Image Lab software for ChemiDoc XRS system (Bio-Rad).
Primary antibodies for western blotting include anti-WRN (Santa Cruz Biotechnology, Inc.), anti-Progerin (Santa Cruz Biotechnology, Inc.), anti-P21 (Cell Signaling Technology, Inc.), anti-LAP2β (BD Bioscience, Inc.), anti-HP1α (Cell Signaling Technology, Inc.), anti-Actin (Santa Cruz, Inc.), anti-Lamin B1 (Abcam, Inc.), anti-P16 (BD Bioscience, Inc.), anti-H3K9me3 (Abcam, Inc.), anti-β-Tubulin (Santa Cruz, Inc.).
Wu Z., Zhang W., Song M., Wang W., Wei G., Li W., Lei J., Huang Y., Sang Y., Chan P., Chen C., Qu J., Suzuki K., Belmonte J.C, & Liu G.H. (2018). Differential stem cell aging kinetics in Hutchinson-Gilford progeria syndrome and Werner syndrome. Protein & Cell, 9(4), 333-350.
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