Cyclohexane

Cedar pollen extract (PE) and pollen extract-galactomannan conjugate (PE-GM) were purchased from Wako Filter Technology Company (Tokyo, Japan). Fluorescein-4-isothiocyanate (FITC) was purchased from Dojindo (Kumamoto, Japan). Cyclohexane and isopropyl myristate (IPM) were obtained from Wako Pure Chemical Industries (Kyoto, Japan) and Tokyo Chemical Industry (Tokyo, Japan), respectively. A surfactant sucrose laurate (L-195) was kindly provided by Mitsubishi–Kagaku Foods (Tokyo, Japan). RPMI-1640 medium, fetal bovine serum (FBS), antibiotic-antimycotic solution, and Imject Alum were from Thermo Fisher Scientific (Waltham, MA, USA). Histamine dihydrochloride was provided by Nacalai Tesque (Kyoto, Japan). Biotin-conjugated Cry j 1 was obtained from Funakoshi (Tokyo, Japan). Horseradish peroxidase-labeled rabbit anti-mouse IgG 1, and IgG 2a were obtained from Rockland Immunochemicals (Gilbertsville, PA, USA). All other reagents used in the experiments were of analytical grade.

NaYF₄ NPs doped with Yb³⁺, Ho³⁺, and Er³⁺ ions were prepared by a thermal decomposition method reported elsewhere^{45 (link),46 (link)} with minor modifications. Yttrium chloride hexahydrate (Sigma-Aldrich, St Louis, MO, USA) (0.78 mmol), ytterbium(III) chloride hexahydrate (Wako Pure Chemical Industries, Osaka, Japan) (0.20 mmol), holmium(III) chloride hexahydrate (Wako Pure Chemical Industries) (0.03 mmol), and erbium(III) chloride hexahydrate (Wako Pure Chemical Industries) (0.005 mmol) were mixed in a solvent composed of distilled water (0.67 mL), OA (14 mL; Sigma-Aldrich), and 1-octadecene (30 mL; Wako Pure Chemical Industries). After stirring at 160 °C for 60 min under a nitrogen atmosphere, the mixture was cooled to room temperature, and a solution of ammonium fluoride (NH₄F, 4 mmol; Kanto Chemicals, Tokyo, Japan) and sodium hydroxide (NaOH, 2.5 mmol; Wako Pure Chemical Industries) in methanol (7 mL; Wako Pure Chemical Industries) was slowly added to the reaction mixture. After stirring, methanol in the mixture was removed by evaporation. Finally, the mixture was heated at 310 °C for 60 min under a nitrogen atmosphere. Finally, the NaYF₄ NPs collected by precipitation were purified by centrifugal washing (20000 g, 10 min, × 3) with ethanol (Wako Pure Chemical Industries) and dispersed in cyclohexane (Wako Pure Chemical Industries).

R848 was purchased from Enzo life science (Farmingdale, NY). Cyclohexane and Rhodamine‐dioleyl phosphatidylethanolamine (Rho‐DOPE) were purchased from Wako Pure Chemical Industries (Kyoto, Japan) and Avanti Polar Lipids (Alabaster, AL), respectively. Isopropyl myristate (IPM) and 5‐carboxyfluorescein (5‐CF) N‐succinimidyl ester were purchased from Tokyo Chemical Industry (Tokyo, Japan). Cedar Pollen Extract‐Cj was supplied by Cosmo Bio (Tokyo, Japan), and Imject Alum from Thermo Scientific (Waltham, MA). Cry j 1 and Biotin‐conjugated Cry j 1 were obtained from Hayashibara (Okayama, Japan). Histamine dihydrochloride was provided by Nacalai Tesque (Kyoto, Japan). Yucatan micropig skin was purchased from Charles River (Burlington, MA). A surfactant sucrose laurate (L‐195) was kindly supplied by Mitsubishi‐Kagaku Foods (Tokyo, Japan).

Cerium
(III) chloride heptahydrate (CeCl₃·7H₂O),
oleic acid, deuterium oxide (D₂O), and amyloid beta (16–20)
peptide [Aβ_16–20; Ac-Lys-(Me)Leu-Val-(Me)Phe-Phe-NH₂] were purchased from Sigma-Aldrich Co. (St Louis, MO, USA),
and
1-octadecene was purchased from Tokyo Chemical Industry Co., Ltd.
(Tokyo, Japan). The N-methylated form of Aβ_16–20 is a commercially available good model for the investigation with
increased stability; however, possible changes in the peptide conformation
and aggregation state associated with a possible increase in hydrophobicity
should be noted. Sodium hydroxide, ammonium chloride (NH₄Cl), sodium nitrate (NaNO₃), sodium chloride (NaCl), methanol,
ethanol, hexane, cyclohexane, and 20% deuterium chloride solution
(DCl) (5.34 mol/L) in D₂O were purchased from Fujifilm
Wako Pure Chemical Co. (Osaka, Japan). Ammonium fluoride was purchased
from Kanto Chemical Co., Inc. (Tokyo, Japan). All reagents were used
without further purification.

All reagents and solvents were commercially available. Sodium tetrachloropalladate(II) (Na₂PdCl₄, 98%), polyvinylpyrrolidone (PVP, M_w ≈ 55,000), ascorbic acid (AA, 99 + %), potassium bromide (KBr, ≥ 99%), oleylamine (OAm, 80–90%), 1-octadecene (ODE, 90%), indium(III) chloride (InCl₃, 98%), indium powder (In, >99.99%), eicosane (99%), ammonium hydrogen solution (NH₃aq, 28–30%), tetraethyl orthosilicate (TEOS, 98%) and polyoxyethylene nonylphenylether (IGEPAL@CO-520, M_w = 441) were purchased from Sigma-Aldrich. Iron pentacarbonyl (Fe(CO)₅, 95%) was purchased from Kanto Chemical Corporation. Oleic acid (OA, > 85%) was purchased from Tokyo Chemical Industry. Lead (II) acetate trihydrate (Pb(OAc)₂·3H₂O), ethanol, acetone, n-hexane, chloroform, and cyclohexane were purchased from Wako Pure Chemical Industries. Boron nitride (BN, > 99.5%) powder was purchased from Nacalai Tesque. Chemical reagents were used as received without further purification except for the synthetic process of Pd–Pb@FeO_y NPs.