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Human il 28b quantikine elisa kit

Manufactured by R&D Systems
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The Human IL-28B Quantikine ELISA Kit is a quantitative sandwich enzyme immunoassay designed to measure human interleukin-28B levels in cell culture supernatants, serum, and plasma. The assay employs an antibody specific for human IL-28B coated on a microplate. Standards and samples are pipetted into the wells, and any IL-28B present is bound by the immobilized antibody.

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Lab products found in correlation

Synergy neo plate reader, by Agilent Technologies (2 mentions) Human ifn gamma elisa ready set go, by Thermo Fisher Scientific (2 mentions) Human tnf alpha elisa ready set go, by Thermo Fisher Scientific (2 mentions) Verikine human ifn alpha elisa kit, by PBL Assay Science (2 mentions) Human il 28a duoset elisa, by R&D Systems (2 mentions) Human il 29 duoset elisa, by R&D Systems (2 mentions) Mouse ifn gamma elisa ready set go, by Thermo Fisher Scientific (2 mentions) Anti ana and anti dsdna elisas, by Alpha Diagnostic (2 mentions) Verikine hs human ifn beta serum elisa kit, by PBL Assay Science (2 mentions) Amicon ultra centrifugal filter, by Merck Group (2 mentions) Mouse tnf alpha quantikine elisa kit, by R&D Systems (2 mentions)

Close spelling variants of this product

Quantikine ELISA kit (1163 citations) Quantikine ELISA (398 citations) ELISAs (144 citations) Quantikine kits (80 citations) IL-28B (5 citations) Quantikines (4 citations) Quantikine Human (2 citations) Human kits (2 citations)

2 protocols using human il 28b quantikine elisa kit

1

Quantification of Cytokine Levels in Cell Cultures

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Cells were treated with DMSO or abemaciclib (500 nM) for 7 days. For the last 24 hours, culture media was replaced with serum free media. Following concentration of conditioned medium using Amicon Ultra centrifugal filters (Millipore), cytokines were analyzed according to the manufacturer’s recommendations: human IFN gamma ELISA Ready-SET-Go! (Affymetrix eBioscience), human TNF alpha ELISA Ready-SET-Go! (Affymetrix eBioscience), Verikine human IFN alpha ELISA kit (PBL assay science), VeriKine-HS human IFN beta serum ELISA kit (PBL assay science), human IL-28B quantikine ELISA kit (R&D Systems), human IL-28A DuoSet ELISA (R&D Systems), and human IL-29 DuoSet ELISA (R&D Systems). Cytokine production by OT-I T cells in OVA/OT-I co-culture assays was measured by mouse IFN gamma ELISA Ready-SET-Go! (Affymetrix eBioscience) and mouse TNF-alpha Quantikine ELISA Kit (R&D Systems) according to the manufacturer’s instructions. Anti-ANA and anti-dsDNA ELISAs (Alpha Diagnostic) were performed on plasma according to the manufacturer’s instructions. Absorbance was measured on a Synergy Neo plate reader (BioTek) using Gen5 software.
Goel S., DeCristo M.J., Watt A.C., BrinJones H., Sceneay J., Li B.B., Khan N., Ubellacker J.M., Xie S., Metzger-Filho O., Hoog J., Ellis M.J., Ma C., Ramm S., Krop I.E., Winer E.P., Roberts T.M., Kim H.J., McAllister S.S, & Zhao J.J. (2017). CDK4/6 inhibition triggers anti-tumor immunity. Nature, 548(7668), 471-475.
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2

Quantification of Cytokine Levels in Cell Cultures

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were treated with DMSO or abemaciclib (500 nM) for 7 days. For the last 24 hours, culture media was replaced with serum free media. Following concentration of conditioned medium using Amicon Ultra centrifugal filters (Millipore), cytokines were analyzed according to the manufacturer’s recommendations: human IFN gamma ELISA Ready-SET-Go! (Affymetrix eBioscience), human TNF alpha ELISA Ready-SET-Go! (Affymetrix eBioscience), Verikine human IFN alpha ELISA kit (PBL assay science), VeriKine-HS human IFN beta serum ELISA kit (PBL assay science), human IL-28B quantikine ELISA kit (R&D Systems), human IL-28A DuoSet ELISA (R&D Systems), and human IL-29 DuoSet ELISA (R&D Systems). Cytokine production by OT-I T cells in OVA/OT-I co-culture assays was measured by mouse IFN gamma ELISA Ready-SET-Go! (Affymetrix eBioscience) and mouse TNF-alpha Quantikine ELISA Kit (R&D Systems) according to the manufacturer’s instructions. Anti-ANA and anti-dsDNA ELISAs (Alpha Diagnostic) were performed on plasma according to the manufacturer’s instructions. Absorbance was measured on a Synergy Neo plate reader (BioTek) using Gen5 software.
Goel S., DeCristo M.J., Watt A.C., BrinJones H., Sceneay J., Li B.B., Khan N., Ubellacker J.M., Xie S., Metzger-Filho O., Hoog J., Ellis M.J., Ma C., Ramm S., Krop I.E., Winer E.P., Roberts T.M., Kim H.J., McAllister S.S, & Zhao J.J. (2017). CDK4/6 inhibition triggers anti-tumor immunity. Nature, 548(7668), 471-475.
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