Edge 3.0 digital microscope

Two hours after the last ablation, before euthanasia, 50 mg/kg of triphenyl tetrazolium chloride was administered intravenously to the animals and further survived the study subjects for 10 to 15 minutes. On termination of the study, animals were euthanized, and gross necroscopy was performed. Histological examination of the ablation lesions was performed using hematoxylin and eosin staining and Masson trichrome staining. At treatment sites, ventricular lesion width and depth were measured based on gross lesion measurement under a Dino-Lite Edge 3.0 digital microscope.

A JSM-7401F field emission scanning electron microscope was used to image LNPs and LNPs photonic crystal samples. An accelerating voltage of 1–2 kV and a working distance of 2–15 mm were used during measurement. Some samples were coated for 60–120 s with gold using a JFC-1200 fine coater before the SEM study. The gold particles added by sputtering are about 5–15 nm in size and they were added to increase the contrast in the images. Annular dark field (ADF) scanning transmission electron microscopy (STEM) images were obtained using a ThermoFisher Themis Z double aberration-corrected TEM operated at 300 kV with a convergence angle of 21 mrad and a dwell time of 3 µs. The material was sectioned to 200 nm thickness using ultramicrotomy using a Leica Ultracut UCT with a 45° diamond knife (Diatome) after the crystalline lignin nanoparticles first had been embedded in Agar low viscosity resin to facilitate sectioning.
A Nikon FN-S2N (Japan) microscope was used to image the rectangular platelets of LNPs and record a movie of the assembly and rearrangement of LNPs during evaporation. A Dino-Lite Edge 3.0 digital microscope was used to image the photonic crystals of LNPs.