A volume of 12 µl of protein solution (see Protein Extraction) was mixed with 1× loading buffer (SDS-Sample buffer, Cat.#BP-111R, Boston BioProducts) for each lane, boiled at 95 °C for 20 min, and electrophoresed on SDS polyacrylamide gels (Any Kd 15-well comb mini-gel, Bio-Rad, Cat # 456-9036). A volume of 3 µl of Precision Plus Protein Dual Xtra Stands (Cat#161-0377, Bio-Rad) was used as ladder to indicate the position of the bands, and then blotted onto nitrocellulose membranes (Biorad, Cat. # 162-0213). Blots were probed with anti-αSmooth Muscle actin antibody (1:400 dilution, Rabbit polyclonal to alpha smooth muscle Actin; Cat. # ab5694, AbCam) and anti-P actin antibody (1:4,000 dilution, Monoclonal Anti-β-Actin antibody produced in mouse; Cat #A1978, Sigma Aldrich) as a loading control followed by Donkey Anti-Rabbit (1 to 15,000 dilution, Cat#926-32213, Li-Cor) and Goat Anti-Mouse(1 to 15,000 dilution, Cat#926-68070, Li-Cor) Fluorophore-conjugated secondary antibodies. Antibody-antigen complexes were visualized using an Odyssey Detection system (Li-Cor, Serial No. ODY-2329) at 700 and 800 nm wavelengths. The densities of the bands were analyzed by Image J software.
Precision plus protein dual xtra stands
Manufactured by Bio-Rad
The Precision Plus Protein Dual Xtra Stands are designed to hold and support Precision Plus Protein Dual Color Standards. The stands provide a stable platform for the standards during use in electrophoresis applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using precision plus protein dual xtra stands
1
SDS-PAGE and Western Blot Analysis
2
SDS-PAGE and Western Blot Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A volume of 12 µl of protein solution (see Protein Extraction) was mixed with 1× loading buffer (SDS-Sample buffer, Cat.#BP-111R, Boston BioProducts) for each lane, boiled at 95 °C for 20 min, and electrophoresed on SDS polyacrylamide gels (Any Kd 15-well comb mini-gel, Bio-Rad, Cat # 456-9036). A volume of 3 µl of Precision Plus Protein Dual Xtra Stands (Cat#161-0377, Bio-Rad) was used as ladder to indicate the position of the bands, and then blotted onto nitrocellulose membranes (Biorad, Cat. # 162-0213). Blots were probed with anti-αSmooth Muscle actin antibody (1:400 dilution, Rabbit polyclonal to alpha smooth muscle Actin; Cat. # ab5694, AbCam) and anti-P actin antibody (1:4,000 dilution, Monoclonal Anti-β-Actin antibody produced in mouse; Cat #A1978, Sigma Aldrich) as a loading control followed by Donkey Anti-Rabbit (1 to 15,000 dilution, Cat#926-32213, Li-Cor) and Goat Anti-Mouse(1 to 15,000 dilution, Cat#926-68070, Li-Cor) Fluorophore-conjugated secondary antibodies. Antibody-antigen complexes were visualized using an Odyssey Detection system (Li-Cor, Serial No. ODY-2329) at 700 and 800 nm wavelengths. The densities of the bands were analyzed by Image J software.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!