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S311 10

Manufactured by Dojindo Laboratories

The S311-10 is a product offered by Dojindo Laboratories. It is a laboratory equipment designed for a core function, but a detailed description while maintaining an unbiased and factual approach is not available.

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2 protocols using s311 10

1

Oxidative Stress Biomarkers in Ischemic Injury

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Blood samples were collected at 24 h after reperfusion and plasma was isolated. The creatine kinase (CK, MAK116, Sigma) and lactate dehydrogenase (LDH, MAK066, Sigma) level in plasma were measured according to the manufacturer's instructions. Ventricles were crushed to a powder using liquid nitrogen and homogenized in saline with the weight/volume ratio of 1:10. After centrifuging for 10 min at 3500 rpm, the supernatants were withdrawn for SOD (S311–10, Dojindo Molecular Technologies) and MDA (MAK085, Sigma) measurement according to the manufacturer's instructions. Bradford protein assay was performed to determine the protein concentration.
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2

Colorimetric SOD-like Activity Assay

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The SOD-like activity assays of N-PCNSs were carried out at room temperature by employing a commercial colorimetric SOD assay kit (S311-10) from Dojindo Molecular Technologies. The assay was performed according to the manufacturer’s instructions, and the SOD-like activity of a series concentrations of N-PCNSs was expressed as the percentage inhibition of the competitive WST reaction with superoxide by natural SOD enzyme.
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