Lipolysis rate was measured as described previously [41 (link)]. Briefly, 0.2 g adipose tissues were minced and added into a N-tris-(hydroxymethyl)-methyl-2-aminoethanesulfonic acid (25 mM) buffer containing isoproterenol (1 μM). A glycerol detection kit (Randox, Amtrim, UK) was used to measure the glycerol levels in samples after 1, 2, and 3 h of incubation at 37 °C and the absorbance was recorded at 520 nm. The lipolysis rate was expressed by nano-moles glycerol/gram adipose tissue/h.
Glycerol detection kit
Manufactured by Randox
Sourced in United Kingdom
The Glycerol detection kit is a laboratory equipment product designed to quantitatively detect and measure the concentration of glycerol in a given sample. It provides a reliable and accurate method for the analysis of glycerol content.
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Lab products found in correlation
2 protocols using glycerol detection kit
1
Adipose Tissue Lipolysis Measurement
2
Measuring Adipose Tissue Lipolysis and LPL Activity
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Lipolysis rate was determined by the measurement of glycerol levels as described previously [15 (link)]. A glycerol detection kit (Randox, Antrim, UK) was used to assay the levels of glycerol in samples. The nano-moles glycerol/gram adipose tissue/h was used to express the lipolysis rate.
LPL activity was detected by measurement of p-nitrophenol formation as described previously [15 (link)]. The absorbance at 400 nm was used for detection.
LPL activity was detected by measurement of p-nitrophenol formation as described previously [15 (link)]. The absorbance at 400 nm was used for detection.
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