A total number of 96 female severe combined immunodeficiency (SCID) mice (CB17/Icr-Prkdcscid/lcrlcoCrl) were obtained from a commercial breeder (Charles River Wiga, Sulzfeld, Germany) at 4 weeks of age. Mice were kept in groups of 6 in individually ventilated cage systems (Tecniplast, Hohenpeißenberg, Germany) and had an adaptation period of 2 weeks. Mice were subcutaneously xenotransplanted with DH82 cells as previously described [32 (link),34 (link)]. Briefly, 3.0 ∗ 106 DH82 cells in 100 µL FBS- and NEAA-free medium were injected once into the subcutis of each animal’s left flank using single-use pen needles (Omnican® F, B. Braun, Melsungen, Germany). Subsequently, tumor development was monitored every second to the third day by measuring tumor width and length with an analog vernier precision caliper (C. Schulz measuring instruments, Kloster Lehnin, Germany). Tumor volume was calculated as ((shortest diameter2 × longest diameter)/2) [61 (link)]. Furthermore, total body weight was determined.
Omnican f
Manufactured by B. Braun
Sourced in Germany
The Omnican® F is a medical device designed for the administration of insulin and other injectable medications. It features a fine and thin needle for subcutaneous injection. The core function of the Omnican® F is to provide a convenient and reliable method for delivering measured doses of medications to patients.
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Lab products found in correlation
4 protocols using omnican f
1
Xenotransplantation of DH82 Cells in SCID Mice
2
Galleria mellonella Infection Model
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Larvae of the greater wax moth G. mellonella were obtained from Reptilienkosmos (Niederkrüchten, Germany). For infection experiments, S. pyogenes strains were grown overnight in THY, washed twice in a 0.9% NaCl solution, and suspended in 0.9% NaCl to a final concentration of 1–3 × 108 CFU/mL. Larvae with a weight of 150–200 mg were infected with 1–3 × 106 CFU/larva. Bacteria were injected into the hemocoel of the larvae between the last two pairs of pro-legs using a microapplicator (World Precisions Instruments, Sarasota, FL) and a fine dosage syringe (Omnican F; B. Braun, Melsungen, Germany; 0.01–1 mL, 0.30 × 12 mm). As mock control, 0.9% NaCl was injected. For CPP-PNA treatment, larvae were injected 30 min postinfection with 4 nmol CPP-PNA/larva. Larvae were incubated for 7 days, and survival was monitored daily.46 (link)
3
Colorectal Liver Metastasis Induction in Rats
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CC531 rat colon carcinoma cells (CLS, Heidelberg, Germany), which are syngeneic to WAG/Rij rats, were cultured as described in previously published studies [19 (link)]. For the induction of colorectal liver metastases, the rats were positioned in supine position on an electronically regulated heating pad, which adjusted the body temperature at 37 °C. Under isoflurane anesthesia, we conducted a median laparotomy and subcapsular implantation of syngeneic 5 × 105 CC531 cells (in 50 µL phosphate buffered saline) on the lower surface of the left liver lobe using a 30G × 1/2” needle (Omnican® F, B. Braun Melsungen AG, Melsungen, Germany). Then, the liver was repositioned anatomically into the peritoneal cavity and the laparotomy was closed with a one-layer running Vicryl 4-0 suture (Ethicon/Johnson & Johnson Medical Ltd., Livingston, UK).
4
Galleria mellonella Infection Model
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Larvae of the greater wax moth Galleria mellonella were obtained from Reptilienkosmos (Niederkrüchten, Germany). Infection experiments were carried out as described elsewhere [24 (link)]. In short, S. pyogenes strains were grown overnight in THY, washed twice in a 0.9% NaCl solution and suspended in 0.9% NaCl to a final concentration of 1.5-2x108 CFU/ml. Larvae with a weight of 150–200 mg were inoculated with 10 μl of this bacterial suspension, resulting in an infection dose of 1.5–2 x 106 CFU/larva. Bacteria were injected into the hemocoel of the larvae between the last pair of legs using a microapplicator (World Precisions Instruments, Sarasota, USA) and a fine dosage syringe (Omnican-F, 0.01 ml–1 ml, 0.30x12 mm, B. Braun AG, Melsungen, Germany). As a control, larvae were mock inoculated with 10 μl of a 0.9% NaCl solution. Survival of the larvae was observed for seven days. Larvae were regarded dead when they did not move upon repeated physical stimulation with tweezers.
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