Lysophosphatidic acid

Manufactured by Santa Cruz Biotechnology

Lysophosphatidic acid is a lipid signaling molecule that regulates various cellular processes. It is a naturally occurring phospholipid derivative that can be isolated and purified for use in research applications.

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Lab products found in correlation

Thapsigargin, by Thermo Fisher Scientific (1 mentions) Lovastatin, by Selleck Chemicals (1 mentions) Dobutamine, by Merck Group (1 mentions) Simvastatin, by Selleck Chemicals (1 mentions) Lapatinib, by Selleck Chemicals (1 mentions) Doxycycline, by Merck Group (1 mentions) Epidermal growth factor (egf), by Merck Group (1 mentions) Phorbol 12 myristate 13 acetate pma, by Merck Group (1 mentions) Phospho pras40, by Cell Signaling Technology (1 mentions) Pras40, by Cell Signaling Technology (1 mentions) Phospho gsk3β, by Cell Signaling Technology (1 mentions) Phospho foxo1, by Cell Signaling Technology (1 mentions) Prolong gold mounting medium with dapi, by Thermo Fisher Scientific (1 mentions) Recombinant human pdgf bb, by R&D Systems (1 mentions) Recombinant human c5a, by Thermo Fisher Scientific (1 mentions) Eea1 antibody, by BD (1 mentions) Anti mouse alexa 488, by Thermo Fisher Scientific (1 mentions) Lamp 1 antibody, by Abcam (1 mentions) 4e bp1, by Cell Signaling Technology (1 mentions) Foxo3a, by Cell Signaling Technology (1 mentions)

4 protocols using lysophosphatidic acid

Investigating Signaling Pathways in MCF10A Cells

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Thapsigargin (TG): Alfa Aesar—#J62866. One millimolar DMSO stock. Cells were treated at 1 μM and imaged immediately.
Src inhibitor (PP1): Cayman Chemical Company—#14244. Ten millimolar DMSO stock. Cells were treated at 10 μM and imaged after 1 hr for YAP fluctuation experiments or immediately for transcription experiments.
PKC inhibitor (Go 6976): Tocris, #2253. One millimolar DMSO stock. Cells were treated at 1 μM for 2 h before being treated by TG.
Serum starvation: MCF10A cells were seeded on MATTEK dishes (35 mm, No. 1.5 glass) and grown in complete media for 3 days before serum starvation in DMEM/F12 without other supplements for 20 h. These cells were then either replenished with serum or treated with 1 µM lysophosphatidic acid (Santa Cruz Biotechnology).

Franklin J.M., Ghosh R.P., Shi Q., Reddick M.P, & Liphardt J.T. (2020). Concerted localization-resets precede YAP-dependent transcription. Nature Communications, 11, 4581.

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Cellular Signaling Pathway Modulation

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NRG1 (Sigma), EGF (Sigma), lapatinib (Selleck), dobutamine (Sigma), lysophosphatidic acid (LPA, Santa Cruz), doxycycline (Sigma), erlotinib (LC Labratories), simvastatin (Selleck), lovastatin (Selleck), Phorbol 12-myristate 13-acetate (PMA, Sigma).

Haskins J.W., Nguyen D.X, & Stern D.F. (2014). Neuregulin 1-activated ERBB4 interacts with YAP to induce Hippo pathway targetgenes and promote cell migration. Science signaling, 7(355), ra116.

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Comprehensive Antibody Toolkit for Cellular Signaling

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The following antibodies were purchased from Cell Signaling Technology: Akt(#4691), Phospho-Akt(Thr308)(#4056), Phospho-Akt(Ser473)(#4060), GAPDH, GSK-3β(#9315), Phospho-GSK-3β(#9323), FoxO1(#2880), Phospho-FoxO1 (Thr24)/FoxO3a(Thr32)/FoxO4(Thr28)(#2599), PRAS40(#2691), Phospho-PRAS40 (Thr246)(#2997), 4E-BP1(#9644), p70 S6K(#2708), Phospho-p70 S6K (Thr389)(#9234). SQSTM1/p62 (D5E2)(#8025), LC3B(#12513). PARP(#9532), Caspase-3(#9665), Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204)(#4377), p44/42 MAPK (Erk1/2) (#4695), Phospho-NF-ΚB P65 (Ser 536)(#3033), NF-ΚB P65(#4764). Lysophosphatidic Acid (#sc-201053, Santa Cruz), Recombinant Human PDGF-BB (#220-BB, R&D), Recombinant Human C5a (Peprotech, #300-70), goat anti-IgM (#5C07615, Meridian). LC3B antibody (#3868, CST). Tubulin antibody (#8035, Santa Cruz). EEA1 antibody (#610456, BD Bioscience) and LAMP1 antibody (#24170, Abcam). Secondary antibodies, Alexa488-anti-mouse, Alexa594-anti-rabbit and Prolong gold mounting medium with DAPI were purchased from Invitrogen.

Liu X., Wang A., Liang X., Liu J., Zou F., Chen C., Zhao Z., Deng Y., Wu H., Qi Z., Wang B., Wang L., Liu F., Xu Y., Wang W., Fernandes S.M., Stone R.M., Galinsky I.A., Brown J.R., Loh T., Griffin J.D., Zhang S., Weisberg E.L., Zhang X., Liu J, & Liu Q. (2016). Simultaneous inhibition of Vps34 kinase would enhance PI3Kδ inhibitor cytotoxicity in the B-cell malignancies. Oncotarget, 7(33), 53515-53525.

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Cell Line Stimulation with LPA and PMA

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Cell lines were cultured and stimulated with LPA or PMA, as described elsewhere^{8 (link)}. A375 and Hs294T cell lines were obtained from the ATCC, WM1341D, and WM9 cell lines were from Rockland Immunochemicals, Inc., whereas SK-MEL-28 were bought at CLS Cell Lines Service GmbH. Cell lines were subcultured twice per week. Before Phorbol-12-Myristate-13-Acetate (PMA, Santa Cruz Biotechnology Inc.) or Lysophosphatidic Acid (LPA, Santa Cruz Biotechnology Inc.) treatment, the cells were starved for 24 h in medium without FBS (fetal bovine serum). Cells were stimulated with 1 µM LPA for 10 min or with 100 nM PMA for 5 min at 37 °C in 5% CO₂.

Malek N., Michrowska A., Mazurkiewicz E., Mrówczyńska E., Mackiewicz P, & Mazur A.J. (2021). The origin of the expressed retrotransposed gene ACTBL2 and its influence on human melanoma cells’ motility and focal adhesion formation. Scientific Reports, 11, 3329.

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