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AICDA is an enzyme that catalyzes the deamination of cytidine to uridine in single-stranded DNA. It plays a role in somatic hypermutation and class-switch recombination processes during B-cell development.

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Lab products found in correlation

Hdac1, by Thermo Fisher Scientific (1 mentions) P14arf, by Santa Cruz Biotechnology (1 mentions) P16 clone jc8, by Santa Cruz Biotechnology (1 mentions) Setd7, by Cell Signaling Technology (1 mentions) Smad3, by Thermo Fisher Scientific (1 mentions) α tubulin, by Merck Group (1 mentions) β actin, by Santa Cruz Biotechnology (1 mentions) Flag tag (flag), by Merck Group (1 mentions) Supersignal west pico plus chemiluminescent substrate, by Thermo Fisher Scientific (1 mentions) β actin, by Merck Group (1 mentions)

2 protocols using aicda

1

Immunoblotting and ChIP Antibody Protocols

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The following antibodies were used for immunoblotting; p16 (clone JC8, sc-56330, Santa Cruz Biotechnology), p14 ARF (Santa Cruz sc-8613), beta Actin (Santa Cruz, sc-69879), Flag (Sigma, F1804), AICDA (Cell signaling, #4975), SMAD3 (Invitrogen, 51–1500), Rb1 (cell signaling, #9309), SETD7 (Cell signaling, #2825), and alpha Tubulin (Sigma, T6074), and chromatin immunoprecipitation; CtBP1 (EpiGentek, #A2705), HDAC1 (Thermo Fisher PA1-860), and p300 (Bethyl A300-358A) and p53 (Sant Cruz, sc126).
Ohashi M., Hayes M., McChesney K, & Johannsen E. (2021). Epstein-Barr virus nuclear antigen 3C (EBNA3C) interacts with the metabolism sensing C-terminal binding protein (CtBP) repressor to upregulate host genes. PLoS Pathogens, 17(3), e1009419.
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2

Quantification of Immune Signaling Proteins

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Whole-cell lysates were obtained from spleen or purified splenic B cells
and bone marrow using the NP40 lysis buffer (1% NP40, 50mM Tris pH8.0, 150mM
NaCl, 10% glycerol, 1mM EDTA, with protease/phosphatase inhibitors)[27 (link)]. P roteins were separated by
SDS/polyacrylamide gel electrophoresis (SDS-PAGE), transferred to polyvinylidene
difluoride (PVDF) membrane in 1X Tris-glycine buffer (3.03g/L Tris-base, 14.4g/L
glycine, 20% methanol), as reported earlier[28 ]. For detection of relevant proteins, the following antibodies
were used: IRF8 (Santa Cruz Biotechnology, cat# sc-365042, 1:1000), AICDA (Cell
Signaling Technology, cat# 4975, 1:1000), BCL6 (Santa Cruz Biotechnology, cat#
sc-858 or Cell Signaling Technology, cat# 4242, 1:1000), β-actin
(Sigma-Aldrich, cat#A2228, 1:20000). Goat anti-rabbit or mouse IgG-HRP conjugate
were used as secondary antibodies (Bio-Rad Laboratories, cat#1706515 and
cat#1706516, 1:5000 or 1:10000, respectively). The proteins were visualized
using the SuperSignal® West Pico PLUS Chemiluminescent
Substrate (Thermo Scientific, cat# 34580), and manual film development or
digital images (FluorChemR system, ProteinSimple).
Qiu Z., Holder K.N., Lin A.P., Myers J., Jiang S., Gorena K.M., Kinney M.C, & Aguiar R.C. (2020). Generation and Characterization of the Eμ-Irf8 mouse model. Cancer genetics, 245, 6-16.
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