During RF experiments, mice were fed normal chow (Ssniff R/M-H, Ssniff Spezialdiäten GmbH, Soest, Germany; 9% kcal from fat) or canola oil-enriched chow (COEC) (powdered normal chow mixed with canola oil, 4∶1 wt/wt; 47% kcal from fat). When using COEC, mice were allowed to habituate to the newly introduced diet before starting the actual experiment.
R m h
Manufactured by Ssniff
Sourced in Germany
The Ssniff R/M-H is a laboratory equipment designed for the preparation and analysis of samples. It serves as a homogenizer and mixer, capable of efficiently processing a variety of sample types.
Automatically generated - may contain errors
Lab products found in correlation
Fvb n mice, by Charles River Laboratories (1 mentions)
Wild type c57bl 6n mice, by Taconic Biosciences (1 mentions)
Cd 1 mice, by Charles River Laboratories (1 mentions)
C57bl 6n mice, by Charles River Laboratories (1 mentions)
Rompun, by Bayer (1 mentions)
Ketalar, by Pfizer (1 mentions)
Nmri mice, by Janvier Labs (1 mentions)
To901317, by Cayman Chemical (1 mentions)
Balb c mice, by Janvier Labs (1 mentions)
25 protocols using r m h
1
Effects of Canola Oil-Enriched Chow on Mice
2
Transgenic Alzheimer's Mouse Models
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Female wild-type mice, female transgenic mice expressing mutated human amyloid precursor protein (APP) and presenilin 1 (PS1) under control of the Thy1-promoter (APPKM670/671NL, PSL166P) (referred to as APP/PS1-21 mice) [30 (link)] and female and male Abcc1(−/−) mice, all with a C57BL/6J genetic background, were generated at the KPM Radium Hospital (Oslo, Norway). At the start of the experiment, mean age was 158 ± 7 days (range: 149–167 days) for wild-type mice, 158 ± 0 days (range: 158–159 days) for APP/PS1-21 mice and 135 ± 37 days (range: 84–161 days) for Abcc1(−/−) mice. Animals were housed in type III IVC cages under controlled environmental conditions (22 ± 3 °C, 40–70% humidity, 12-hour light/dark cycle) and had free access to standard laboratory rodent diet (ssniff R/M-H, ssniff Spezialdiäten GmbH, Soest, Germany) and water. An acclimatization period of at least one week was allowed before the animals were used in the experiments. The study was approved by the national authorities (Amt der Niederösterreichischen Landesregierung, approval number: LF1-TVG-48/006-2015, date of approval: 30 March 2015) and study procedures were in accordance with the European Communities Council Directive of 22 September 2010 (2010/63/EU). The animal experimental data reported in this study are in compliance with the ARRIVE (Animal Research: Reporting in Vivo Experiments) guidelines.
3
Transgenic Mouse Model Experiments
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For the imaging experiments
female C57BL/6N wild-type mice (Taconic, Skensved, Denmark) and female
homozygous Abcb1a/1b(−/−) and heterozygous Abcb1a/1b(+/−) mice backcrossed to the C57BL/6 background (N >10) were used. For the other experiments female wild-type FVB/N mice
(Charles River, Sulzfeld, Germany) and female Abcb1a/1b(−/−) mice with an FVB genetic background
(Taconic, Germantown, NY, USA) were used. All animals were housed
in groups under individual ventilated cage conditions in polysulfon
type III cages. The environmental conditions were as follows: temperature,
22 ± 3 °C; humidity, 40% to 70%; and a 12-h light/dark cycle
(lights on at 6:00) with free access to standard laboratory rodent
diet (ssniff R/M-H, ssniff Spezialdiäten GmbH, Soest, Germany)
and tap water. An acclimatization period of at least 1 week was allowed
before the animals were used in the experiments. All animal experiments
were approved by the national authorities (Amt der Niederösterreichischen
Landesregierung), and all study procedures were performed in accordance
with the European Communities Council Directive of September 22, 2010
(2010/63/EU). All efforts were made to comply with the 3Rs principle
in this study.
female C57BL/6N wild-type mice (Taconic, Skensved, Denmark) and female
homozygous Abcb1a/1b(−/−) and heterozygous Abcb1a/1b(+/−) mice backcrossed to the C57BL/6 background (N >10) were used. For the other experiments female wild-type FVB/N mice
(Charles River, Sulzfeld, Germany) and female Abcb1a/1b(−/−) mice with an FVB genetic background
(Taconic, Germantown, NY, USA) were used. All animals were housed
in groups under individual ventilated cage conditions in polysulfon
type III cages. The environmental conditions were as follows: temperature,
22 ± 3 °C; humidity, 40% to 70%; and a 12-h light/dark cycle
(lights on at 6:00) with free access to standard laboratory rodent
diet (ssniff R/M-H, ssniff Spezialdiäten GmbH, Soest, Germany)
and tap water. An acclimatization period of at least 1 week was allowed
before the animals were used in the experiments. All animal experiments
were approved by the national authorities (Amt der Niederösterreichischen
Landesregierung), and all study procedures were performed in accordance
with the European Communities Council Directive of September 22, 2010
(2010/63/EU). All efforts were made to comply with the 3Rs principle
in this study.
4
Wistar Rat Experimental Protocol
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In the present study, altogether 20 adult SPF male rats of Wistar strain (Rattus norvegicus, 339–387 g, 11–14 weeks old), originally from Envigo (formerly Harlan), Italy (purchased from Anlab, ltd., Prague, Czech Republic, at delivery weight of 275–349 g, 9–12 weeks old), were used. During acclimatization period (at least 7 days before experiment), the animals were housed in animal quarters (Center for Work with Laboratory Animals of the Palacky University, Medical Faculty in Olomouc, Czech Republic) in standard plastic cages with dust-free bedding (sawdust), 2 rats per cage, under controlled environmental conditions with free access to tap water and standard rat chow (pellets from Ssniff® R/M-H, Ssniff Spezialdiäten GmbH, Soest, Germany).
5
Transgenic Mouse Models for Neuroinflammation
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Twelve week old female wild-type (WT; C57BL/6 J), CCR2 knockout (CCR2−/−)43 (link), Cx3cr1GFP/+ 44 (link), Cx3cr1CreERT2 45 (link), Rosa26-LSL-YFP46 (link), PLPCreERT2 47 (link), Rpl22HA 24 (link), PLPCreERT2 47 (link), iDTR48 (link) and their littermate controls were used. All lines were bred and maintained at the KU Leuven animal facility on a 12:12-h light-dark cycle and had ad libitum access to tap water and commercially available chow (ssniff® R/M-H, ssniff Spezialdiäten GmbH). All experimental procedures were approved by the Animal Care and Animal Experiments Ethical Committee of KU Leuven.
6
Chronic Selenium Supplementation in Alzheimer's Disease Mouse Model
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Triple-transgenic AD mice (3xTg-AD; C57BL/6 × 129 background) harboring the human PS1 (M146V), APPSwe (K670N/M671L) and tau (P301L) transgenes and wild-type (WT) mice3, were bred at the Universitat Autònoma de Barcelona (UAB). All mice were females (3xTg-AD = 24 and WT = 21) housed in groups of 3–6 per cage in standard laboratory conditions, including a 12 h light/dark cycle and ad libitum access to water and food pellets (Brand: ssniff® R/M-H, containing 0.3 mg Se/Kg; ssniff Spezialdiäten GmbH, Germany). We treated 8 month-old mice with vehicle (3xTg-AD, n = 9; WT, n = 11) or 12 μg/ml sodium selenate (Se; Sigma-Aldrich/Merck, Darmstadt, Germany) (3xTg-AD, n = 15, one died during testing; WT, n = 10) added to the drinking water ad libitum, and continued throughout behavioral testing. Chronic treatment with Se-Met (6 μg/ml) in drinking water was shown previously to elevate Se in the cortex and hippocampus (1.2–1.5 mg/kg) indicating incorporation of Se in the brain20 (link). The mice were approximately 12 months old at behavioral testing, and 13–14 months old at physiological, biochemical and pathological analyses.
7
Standardized Animal Housing Conditions for Behavioral and Binding Experiments
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For the behavioural experiments male CD-1 mice (Charles River, Sulzfeld, Germany) were kept under controlled laboratory conditions with a light/dark cycle of 12:12 (lights on at 06.00 a.m.), temperature 20 ± 2°C, and air humidity between 55 and 60%. The animals had free access to commercial pellets (ssniff R/M-H, ssniff Spezialdiäten GmbH, Soest, Germany) and tap water. The animals were housed in groups of 10 in Macrolon III cages. After arrival, the animals were given a period of 2 weeks for habituation. At the beginning of the experiments the mice were 8 weeks old. The number of animals per group was between 12 and 18.
For the binding experiment male RjHan:WI rats (Janvier, Le Genest-Saint-Isle, France) were used. The rats were kept under controlled laboratory conditions as described above. The animals were housed in groups of 5 in Macrolon IV cages.
The work reported here was conducted in accordance with EC regulations and the National Act on the Use of Experimental Animals (Germany). The protocol was approved by the Saxony-Anhalt Committee on Animal Care (42502-2-1169).
For the binding experiment male RjHan:WI rats (Janvier, Le Genest-Saint-Isle, France) were used. The rats were kept under controlled laboratory conditions as described above. The animals were housed in groups of 5 in Macrolon IV cages.
The work reported here was conducted in accordance with EC regulations and the National Act on the Use of Experimental Animals (Germany). The protocol was approved by the Saxony-Anhalt Committee on Animal Care (42502-2-1169).
8
Acute Liver Damage Pharmacokinetics in Mice
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8–10 weeks old male C57BL/6N mice, weighing 20–25 g (Charles River, Sulzfeld, Germany) were used. The mice were fed ad libitum with ssniff R/M-H, 10 mm standard diet (ssniff, Soest, Germany) and housed at controlled ambient temperature of 25 °C with 12 h day/12 h night cycle. All experiments were performed in accordance with the relevant guidelines and regulations and approved by the local animal ethics committees (institution name: Landesamt fuer Natur, Umwelt und Verbraucherschutz Nordrhein-Westfalen. Application number: 84–02.04.2012.A333). Acute liver damage was induced by a single intraperitoneal injection of CCl4 (1.6 g/kg) diluted in olive oil (1:4). For pharmacokinetic analysis a cocktail of caffeine (5 mg/kg), midazolam (2 mg/kg), torsemide (2 mg/kg), codeine (2 mg/kg), talinolol (1 mg/kg) and pravastatin (20 mg/kg) was injected as intravenous bolus in the tail vein of untreated mice as well as on day one after CCl4 administration. Blood samples were collected in a time-resolved manner (2, 15, 30, 60 and 120 min) after administration of the cocktail from the portal vein, the hepatic vein and the right heart chamber as described before29 (link). Three mice were used for each time point. Subsequently, blood plasma was separated by centrifugation at 10,000 rpm for 10 min and stored at −80 °C until analysis.
9
Food Intake Responses to PrRP Analogs in Mice
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All of the animal experiments followed the ethical guidelines for animal experiments and the Act of the Czech Republic Nr. 246/1992 and were approved by the Committee for Experiments with Laboratory Animals of the Academy of Sciences of the Czech Republic.
Male C57BL/6 mice from Charles Rivers Laboratories (Sulzfeld, Germany) were housed at a temperature of 23°C with a daily 12 h light/dark cycle (lights on at 6:00 am). The mice were given ad libitum water and a standard rodent chow diet Ssniff® R/M-H (Ssniff Spezialdiäten GmbH, Soest, Germany) and housed until three months of age. The mice were fasted overnight (17 h) prior to the food intake experiment and then subcutaneously (SC) injected with 200 μl of either saline or PrRP analogs at doses of 5 mg/kg (all dissolved in saline, n = 5–6). Fifteen minutes after injection, the mice were given weighed food pellets. The pellets were weighed every 30 min for at least 6 h, and the animals had free access to water during the experiment. The results are expressed as grams of food consumed.
Male C57BL/6 mice from Charles Rivers Laboratories (Sulzfeld, Germany) were housed at a temperature of 23°C with a daily 12 h light/dark cycle (lights on at 6:00 am). The mice were given ad libitum water and a standard rodent chow diet Ssniff® R/M-H (Ssniff Spezialdiäten GmbH, Soest, Germany) and housed until three months of age. The mice were fasted overnight (17 h) prior to the food intake experiment and then subcutaneously (SC) injected with 200 μl of either saline or PrRP analogs at doses of 5 mg/kg (all dissolved in saline, n = 5–6). Fifteen minutes after injection, the mice were given weighed food pellets. The pellets were weighed every 30 min for at least 6 h, and the animals had free access to water during the experiment. The results are expressed as grams of food consumed.
10
Transgenic Mouse Model of Alzheimer's Disease
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Female transgenic mice, which express mutated human amyloid precursor protein (APP) and presenilin 1 (PS1) under control of the Thy1-promoter (APPKM670/671NL, PSL166P) (referred to as APP/PS1-21 mice) [32 (link),33 (link)] and age-matched wild-type littermates in a C57BL/6J genetic background were maintained at the University of Oslo and transferred to the imaging site at least three weeks prior to the PET examinations. In total, 50 mice were used in the experiments. All animals were housed in groups of 3–5 animals in individually ventilated (IVC) type III cages under controlled environmental conditions (22 ± 3 °C, 40% to 70% humidity, 12-h light/dark cycle) and had free access to standard laboratory animal diet (ssniff R/M-H, ssniff Spezialdiäten GmbH, Soest, Germany) and water ad libitum. The study was reviewed by the responsible national authorities (Amt der Niederösterreichischen Landesregierung) and approved under study numbers LF1-TVG-48/003-2014 approval date: 08 January 2015 and LF1-TVG-48/044-2019 approval date: 07 May 2019. All study procedures were in accordance with the European Community’s Council Directive of September 22, 2010 (2010/63/EU). The animal experimental data reported in this study are in compliance with the ARRIVE (Animal Research: Reporting In Vivo Experiments) guidelines.
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