Milli q ultrapure water instrument

Waters UPLC (Waters technologies, USA); AB SCIEX Triple TOF 5600 (AB Sciex, USA); Velocity 14 R high-speed refrigerated centrifuge (Dynamic, Australia); Milli-Q ultrapure water instrument (Millipore, USA); Acetonitrile and formic acid (HPLC grade, Merck, Germany); AB Sciex 5600 Triple TOF correction solution (AB Sciex, USA); Epoch Microplate Spectrophotometer (BioTek, USA); BX63 + DP72 Microscope (Olympus, Japan).

Triptolide was provided by Xi’an Haoxuan Biotechnology Co. Ltd. in Xi’an, China. Chitosan oligosaccharide (CSO, MW: about 3000 Da) was purchased from Aladdin (Beijing, China) and purified using dialysis. Hyclone fetal bovine serum, Trypsin, Dulbecco’s Modified Eagle’s Medium (DMEM), and Phosphate Buffered Saline (PBS 1X, sterile) were all obtained from Beijing Solarbio Science and Technology Co. Ltd. (Beijing, China). Dimethyl sulfoxide, acetonitrile and methanol were MS-grade reagents provided by Tedia. The water used in the experiments was prepared by a Milli-Q ultrapure water instrument (Millipore, Bedford, MA, USA). Hepatocytes (HL7702) and mouse pancreatic cancer cells (Panco2) were purchased from American type cultures (Manassas, VA, USA). Other chemicals were of commercially available analytical grade.