Ch201 for total cholesterol

Blood from the heart was collected into coagulant tubes, left to coagulate, and then centrifuged at 3000 × g for 15 min at 4 °C. Serum ALT and AST activities were measured spectrophotometrically using a commercial kit (Human, Wiesbaden, Germany).
Serum and hepatic triglycerides (TG), total cholesterol (TC), low-density lipoprotein (LDL), and high-density lipoprotein (HDL) concentrations were measured using commercial enzymatic kits (Randox® TR213 for triglycerides, Randox® CH201 for total cholesterol, Randox Laboratories Ltd., London, UK).
Liver samples were homogenized in a chloroform/methanol (2:1) solution to a final dilution 20 times the volume of tissues for at least 3 min and extracted with a chloroform/methanol/water (3:48:47) solution [26 (link)].

Blood samples from rat tails were collected at weeks 0, 2 and 9, and centrifuged at 3000 g for 15 min at 4°C. Liver samples from left lateral lobe, median lobe and right lateral lobe were homogenized in chloroform/methanol (2:1) solution and extracted by chloroform/methanol/water (3:48:47) solution. Triglycerides and total cholesterol concentrations in plasma and liver were determined spectrophotometrically at 500 nm using commercial enzymatic kits (Randox® TR213 for triglycerides, Randox® CH201 for total cholesterol, Randox Laboratories Ltd., London, UK).