Blood from the heart was collected into coagulant tubes, left to coagulate, and then centrifuged at 3000 × g for 15 min at 4 °C. Serum ALT and AST activities were measured spectrophotometrically using a commercial kit (Human, Wiesbaden, Germany).
Serum and hepatic triglycerides (TG), total cholesterol (TC), low-density lipoprotein (LDL), and high-density lipoprotein (HDL) concentrations were measured using commercial enzymatic kits (
Randox® TR213 for triglycerides,
Randox® CH201 for total cholesterol, Randox Laboratories Ltd., London, UK).
Liver samples were homogenized in a chloroform/methanol (2:1) solution to a final dilution 20 times the volume of tissues for at least 3 min and extracted with a chloroform/methanol/water (3:48:47) solution [26 (
link)].
Hafez M.M., Hamed S.S., El-Khadragy M.F., Hassan Z.K., Al Rejaie S.S., Sayed-Ahmed M.M., Al-Harbi N.O., Al-Hosaini K.A., Al-Harbi M.M., Alhoshani A.R., Al-Shabanah O.A, & Alsharari S.D. (2017). Effect of ginseng extract on the TGF-β1 signaling pathway in CCl4-induced liver fibrosis in rats. BMC Complementary and Alternative Medicine, 17, 45.