Trpv1 ko

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TRPV1 KO is a specific gene knockout mouse model. It lacks the expression of the TRPV1 (Transient Receptor Potential Cation Channel Subfamily V Member 1) receptor, which is a non-selective cation channel that is primarily expressed in sensory neurons. This model can be used to study the physiological functions of the TRPV1 receptor.

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TRPV1−/− (9 citations) TRPV1 knockout (KO) mice (3 citations)

6 protocols using trpv1 ko

Postmortem Skin and Transgenic Mouse Analysis

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We obtained approval from the National Institutes of Health Office of Human Subjects Research and Protection to obtain and analyze fully deidentified postmortem donor skin specimens provided by the Washington Regional Transplant Community (Falls Church, VA). All animal experiments were approved by the Institutional Animal Care and Use Committee at NC State University (Raleigh, NC) (Institutional Animal Care and Use Committee number 19-167-B) and the National Institutes of Health (Institutional Animal Care and Use Committee number 1402-17). Mice and rats were housed in standard conditions with a 12/12-hour light‒dark cycle with ad libitum access to food and water. Trpv1-KO (stock number 003770), Trpa1-KO (stock number 006401), and C57Bl6/J (stock number 000664) mice were ordered from the Jackson Laboratory (Bar Habor, ME). Trpv1- and Trpa1-KO mice were maintained on the C57Bl6/J background.

Wheeler J.J., Domenichiello A.F., Jensen J.R., Keyes G.S., Maiden K.M., Davis J.M., Ramsden C.E, & Mishra S.K. (2022). Endogenous Derivatives of Linoleic Acid and their Stable Analogs Are Potential Pain Mediators. JID Innovations, 3(2), 100177.

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Murine Nociceptive Behavior Evaluation

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A total of 330 mice were used in this study. All procedures were conducted under a University of Maryland approved Institutional Animal Care and Use Committee protocol in accordance with the NIH Guide for the Care and Use of Laboratory Animals. Adult male C57BL/6 mice, TRPV1 knockout (KO) mice (C57BL/6 background), TRPA1 KO mice (mixed B6;129 background), and their littermate wildtype mice were used as indicated in Results. TRPV1 KO and TRPA1 KO mice were purchased from Jackson Laboratory. For experiments in which facial grimace and wiping behaviors were assessed, 8 to 12 week-old mice were used. For all experiments involving bite force measurements, 12 to 16 week-old mice were used. All animals were housed in a temperature-controlled room under a 12:12 light–dark cycle with access to food and water ad libitum.

Wang S., Brigoli B., Lim J., Karley A, & Chung M.K. (2018). Roles of TRPV1 and TRPA1 in Spontaneous Pain from Inflamed Masseter Muscle. Neuroscience, 384, 290-299.

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Tracing Sensory Neuron Populations

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All animal experiments conformed to APS’s Guiding Principles in the Care and Use of Vertebrate Animals in Research and Training, and to protocols approved by the University Texas Health Science Center at San Antonio (UTHSCSA) Animal Care and Use Committee (IACUC).
All experiments were performed on 8–12-week-old male mice. Following reporter mouse lines were used to delineate small-diameter sensory neurons in this study: TRPV1 KO (stock: 003770), TRPA1 KO (stock: 006401), Rosa26^{LSL-tDTomato/+} (stock: 007914) and vGLUT3^cre/+ (stock: 028534; Jackson Laboratory; Bar Harbor, ME); TRPV1-GFP (GENSAT; UC Davis, CA); CGRP^cre/+-ER; MrgprA3^cre/+ and MrgprD-GFP. Cre-recombinase was induced by 3 (every second day) i.p. injections of 100mg/kg tamoxifen in corn oil. Cre-recombination occurred within 2–3 weeks post last injection of tamoxifen.

Patil M.J., Salas M., Bialuhin S., Boyd J.T., Jeske N.A, & Akopian A.N. (2019). Sensitization of small-diameter sensory neurons is controlled by TRPV1 and TRPA1 association. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(1), 287-302.

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POMC, TRPV1 Neuron Manipulation

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All mouse care and experimental procedures were approved by the Institutional Animal Care Research Advisory Committee of the Albert Einstein College of Medicine. Mice used in these experiments included POMC-Cre (stock # 005965), POMC-eGFP (stock # 009593), TRPV1-Cre (stock # 017769), floxed-stop CRISPR/Cas-9-eGFP mice (stock # 024857), and TRPV1-KO (stock # 003770, the Jackson Laboratory). Both male and female mice of mixed C57BL/6, FVB, and 129 strain backgrounds were used.

Jeong J.H., Lee D.K., Liu S.M., Chua SC J.r., Schwartz G.J, & Jo Y.H. (2018). Activation of temperature-sensitive TRPV1-like receptors in ARC POMC neurons reduces food intake. PLoS Biology, 16(4), e2004399.

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Genetic Mouse Models for Pain Research

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Adult (8–10 weeks) C57BL/6J WT, TRPV1-KO (003770, Jackson Laboratory, San Mateo, California, USA), SHP-1^fl/fl mice (008336, Jackson Laboratory), Ai 14-reporter (B6.Cg-Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze/}J, 007914, Jackson Laboratory), and Na_V1.8-Cre (gift from Rohini Kuner, Heidelberg University, Heidelberg, Germany) mice were used in this study (male C57BL/6J, both male and female transgenic strains). SHP-1–CKO mice were generated by mating Na_V1.8-Cre SHP-1^fl/fl mice. All animals were housed in cages under a 12-hour light/12-hour dark cycle with food and water available ad libitum. Animals were randomly assigned to each group. All the following behavioral testing, electrophysiological recording, and quantification of Western blot experiments described herein were performed by experimenters who were blind with respect to the treatments.

Liu B.L., Cao Q.L., Zhao X., Liu H.Z, & Zhang Y.Q. (2020). Inhibition of TRPV1 by SHP-1 in nociceptive primary sensory neurons is critical in PD-L1 analgesia. JCI Insight, 5(20), e137386.

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Mice Handling and Housing for Experiments

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All experiments involving mice and the procedures used therein were approved by the University of Iowa Institutional Animal Care and Use Committee and were carried out in strict accordance with the NIH Guide for the Care and Use of Laboratory Animals. Every effort was made to minimize the number of mice used and their suffering. Mice (6–10 weeks of age) were housed with food and water ad libitum under a 12-hr light/dark cycle. C57BL/6J, TRPV1 KO (Jackson Labs #003770; C57BL/6J), TRPA1 KO (Jackson Labs #006401; mixed B6;129) and MAFIA (Jackson Labs #005070; C57BL/6J) mice were obtained from The Jackson Laboratory (Farmington, CT).

Warwick C.A., Shutov L.P., Shepherd A.J., Mohapatra D.P, & Usachev Y.M. (2019). Mechanisms underlying mechanical sensitization induced by complement C5a: the roles of macrophages, TRPV1 and CGRP receptors. Pain, 160(3), 702-711.

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