Root tips of six plants from different pots were mixed as a biological replicate. Equal amounts of root tips were collected from each plant. There were three biological replicates for each treatment (total of 18 plants from 18 pots). Total RNA were independently extracted three times from four B and Al combinations using Recalcirtant Plant Total RNA Extraction Kit (Centrifugal column type, Bioteke Corporation, China) according to manufacturer’s instructions. cDNA synthesis and cDNA-AFLP analysis were performed according to Zhou et al. [38 (link)].
Recalcirtant plant total rna extraction kit
Manufactured by BioTeke
Sourced in China
The Recalcirtant Plant Total RNA Extraction Kit is a laboratory product designed to extract total RNA from recalcitrant plant samples. It is a tool for researchers and scientists working with challenging plant materials.
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Lab products found in correlation
4 protocols using recalcirtant plant total rna extraction kit
1
Differentially Expressed Genes in Plant Root Tips
2
Quantifying Gene Expression in Citrus Leaves
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Total RNA were extracted from ~300 mg frozen of leaves (mixed sample from four seedlings, one seedling per pot) using Recalcirtant Plant Total RNA Extraction Kit (Bioteke Corporation, Beijing, China). There were three biological replicates per treatment (a total of 12 seedlings from 12 pots). The sequences of specific primers designed using Primer Primier Version 5.0 (PREMIER Biosoft International, CA, USA), were listed in Table S4 . qRT-PCR was performed with three biological and two technical replicates [86 (link)]. Two Citrus genes: U4/U6 small nuclear ribonucleoprotein PRP31 (PRP31, Cs7g08440.1) and actin (Cs1g05000.1) were used as internal standards and 0.5 μM Cu-treated leaves were used as reference (set as 1).
3
Extracting Total RNA from B-toxic and Control Plants
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Total RNA was extracted from ca. 300 mg of frozen mixed leaves from B-toxic and control plants of C. grandis and C. sinensis using Recalcirtant Plant Total RNA Extraction Kit (Centrifugal column type, Bioteke Corporation, China). There were three biological replicates for each treatment. Leave of 4–5 plants from different pots were mixed as a biological replicate. Equal amounts of leaves were collected from each plant. cDNA synthesis and cDNA-AFLP analysis were performed according to Zhou et al. [44 (link)].
4
Quantitative Analysis of Citrus Root Transcripts
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Root tips of six plants from different pots were mixed as a biological replicate. Equal amounts of root tips were collected from each plant. There were three biological replicates for each treatment. Total RNAs were independently extracted three times from the frozen roots of Al-toxic and control plants using Recalcirtant Plant Total RNA Extraction Kit (Centrifugal column type, Bioteke Corporation, China) according to manufacturer’s instructions. Gene-specific primers were designed using Primer Software Version 5.0 (PREMIER Biosoft International, CA, USA) according to the corresponding sequences of selected proteins in Citrus genome (http://www.phytozome.net/cgi-bin/gbrowse/citrus/ ). The sequences of the F and R primers used are given in Additional file 3 . qRT-PCR analysis was performed according to Zhou et al. [70 (link)]. For the normalization of gene expression, β-tubulin (JN580571) gene was used as an internal standard and the roots from control plants were used as reference sample, which was set to 1.
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