Cd45.1 pe cy7

Manufactured by BioLegend

CD45.1-PE/Cy7 is a fluorescently labeled antibody product manufactured by BioLegend. It is designed for the detection and analysis of cells expressing the CD45.1 antigen, which is commonly used as a marker for certain murine cell populations.

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CD45-PE (78 citations) CD45-PE-Cy7 (69 citations) CD45.1-PE (9 citations) CD45.1-PE/Cy7 (clone A20; (3 citations) Anti-mouse CD45.1-PE/Cy7 (3 citations)

2 protocols using cd45.1 pe cy7

Multi-parameter Immune Cell Profiling

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Cells were incubated 10 min with the TruStain fcX (clone 93) washed and incubated with the antibodies during 20 minutes followed by two washes with PBS. Monoclonal antibodies specific for mouse molecules were purchased from Biolegend: CD3-FITC (clone 17A2), CD3-APC (clone 17A2), CD3-PerCp/Cy5.5 (clone 17A2), CD8-Brillant Violet 421 (clone 53-6.7), CD45-PE (clone 30-F11), CD45-PErCP(clone 30-F11), CD45.1-PE/Cy7 (clone A20), CD45.1-FITC (clone A20), CD103-APC (clone 2E7), CD103-PerCP (clone 2E7), CD69-APC/Cy7 (clone H1.2F3), CD69-APC (clone H1.2F3), CD44-PerCP (clone IM7), IFN-γ-PE (clone XMG1.2), IFN-γ-APC (clone XMG1.2), TNF-α-APC/Cy7 (clone MP6-XT22), CD11b-FITC (clone M1/70), CD207-PE (clone 4C7), XCR1-APC (clone ZET), XCR1-PerCP-Cy5.5 (clone ZET), CD11c-PE/Cy7 (clone N418), MHCII-APC/Cy7 (clone M5/114.15.2), CD24-PerCP-Cy5.5 (clone M1/69), CD80-APC (clone 16-10A1), CD80-PE/Cy7 (clone 16-10A1), CD86 Brilliant Violet 421 (clone GL-1), CCR7-PE/Cy7 (clone 4B12), IL-2-PE/Cy7 (clone JES6-5H4) IL-12/23-APC (clone C15.6), granzyme B-APC (clone GB11) and viability dye Zombie Aqua (ref 423101). Samples were acquired in a FACSCanto II cytometer (BD Bioscience) and data were analyzed using FlowJo version X.0.7 (Tree Star, Inc.). Gating strategies for all flow cytometry experiments are shown in Supplementary Fig. 4.

Menares E., Gálvez-Cancino F., Cáceres-Morgado P., Ghorani E., López E., Díaz X., Saavedra-Almarza J., Figueroa D.A., Roa E., Quezada S.A, & Lladser A. (2019). Tissue-resident memory CD8+ T cells amplify anti-tumor immunity by triggering antigen spreading through dendritic cells. Nature Communications, 10, 4401.

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Neutrophil Phagocytosis of E. coli Bioparticles

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Fluorescein-labeled heat-killed E. coli bioparticles (100 µg) resuspended in PBS were injected into the peritoneal cavity of WT recipient mice that had been previously transplanted with either WT or TAZ-KO fetal liver cells and allowed to engraft and reconstitute for a minimum of 16 weeks. After 3 hours, peritoneal cells were collected by instilling 10 ml of FACS buffer into the peritoneum of a euthanized mouse (21 G syringe) and then removed as much fluid as could be obtained using a syringe affixed to a 16 G needle. The cells were washed and stained with DAPI as well as the following mouse antibodies: CD45.1-PeCy7, CD45.2-PE, CD11b-APC, Ly6C-BV711, Ly6G-APC-Cy7 (BioLegend). Flow cytometry was performed to identify bioparticles engulfed by neutrophils.

Sohn J., Milosevic J., Brouse T., Aziz N., Elkhoury J., Wang S., Hauschild A., van Gastel N., Cetinbas M., Tufa S.F., Keene D.R., Sadreyev R.I., Pu W.T, & Sykes D.B. (2022). A new murine model of Barth syndrome neutropenia links TAFAZZIN deficiency to increased ER stress-induced apoptosis. Blood Advances, 6(8), 2557-2577.

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