7890a gas chromatograph gc

Samples were analyzed by 7890A GC gas chromatograph combined with a 5975C mass spectrometer (Agilent Technologies, Santa Clara, CA, USA) on a DB5-MS capillary column (30 m × 0.25 mm × 0.25 µm with 5% Phenyl methyl siloxane, J & W Scientific, Folsom, CA, USA). GC condition was: helium was the carrier gas and flow rate through the column was 1.5 mL per min. The oven temperature programmed at 40 °C for 1.5 min, then ramped up to 150 °C at 5 °C per minute, after holding there for 13 min, ramped up to 230 °C at 15 °C per minute, and held for 15 min. Mass spectra were obtained at 70 eV.

Air samples for PAH analysis were collected during the entire duration of the exposure (180 min) on Teflo air sampling membranes (PTFE, 37 mm diameter, 2 µm pore size, Pall Corp., Port Washington, NY, USA) followed by XAD-2 tubes (SKC Inc., Dorset, UK). Particle filters and XAD tubes were extracted using dichloromethane analyzed for 32 native PAHs (including the 16 U.S. EPA priority PAHs) and 16 alkylated species. Target compounds were separated on an Agilent 5975C mass spectrometer (MS) coupled to a 7890A gas chromatograph (GC, Agilent Technologies, Santa Clara, CA, USA) [11 ]. Analyses of benzene, toluene, ethyl benzene, m + p xylene, and o-xylene (BTEX) were performed by the Swedish Environmental Institute (IVL). The samples were collected on Tenax^® TA thermal desorption tubes (SKC Inc., Dorset, UK) and analyzed by thermal desorption GC-MS. Target compounds were separated on a non-polar analytical mass spectrometer (TraceGold, TG-1MS, Thermo Fisher Scientific Inc., Franklin, MA, USA) coupled to an ISQ LT (Thermo Fisher Scientific Inc., Franklin, MA, USA) [11 ].

Pomegranate seeds oil was extracted using Soxhlet apparatus following NF NE ISO 659 reference method (AFNOR, 2009 ). Thus, twenty-five grams of each seeds powder genotypes were mixed with 250 mL of n-hexane (99%) as an oil extracting solvent using cellulose extraction thimbles (123 × 43 mm, Whatman International, Brentford, UK). The solvent was evaporated at 40 °C using a rotavapor. The resulting oil samples were stored in darkness at 4 °C until analysis to avoid their oxidation.
The methyl esters of the pomegranate seed oil were prepared in n-heptane (0.12 g/2 mL) with a cold KOH solution (2M) according to standard NF EN ISO 5509. The obtained fatty acid esters were analyzed using an Agilent Technologies 7890A gas chromatograph (GC) equipped with a flame ionization detector (T = 250 °C). The column used was a 60 m × 0.25 mm silica capillary column. The hydrogen inlet pressure of carrier gas was 178 kPa, with 1:70 ratio. The oven temperature program was as follows: 20 min at 210 °C, 210–245 °C at 6 °C/min then 10 min at 245 °C. A volume of 1 mL was injected with a split ratio of 1:50. The fatty acids methyl ester reference standard mixture (C4–C24, FAME Mix 37) was used for used for calibration and for the identification of the FAME by their retention times.