Rid 10a

¹H NMR and ¹³C NMR spectroscopy were performed on Avance DRX 400 MHz. Dimethylformamide (DMF) GPC was conducted in DMF containing 0.10 M LiBr (40 °C, 0.8 mL/min) on a Shimadzu HPLC system equipped with a refractive index detector RID-10A, one Polymer Laboratories PLgel guard column, and two Polymer Laboratories PLgel 5 μm mixed D columns. Calibration was performed using near-monodisperse polystyrene standards. Chromato-grams were processed using the EZStart 7.2 chromatography software. Aqueous GPC was performed in 0.05 M Na₂SO₄ in MQ water with 10% MeOH on a Viscotek GPC_max VE 2001 GPC solvent/sample module equipped with Viscotek TDA 305 triple array detector. Gel electrophoresis was performed using Any kDTM Mini-PROTEAN TGXTM precast gels with SDS-Tris-glycine as running buffer (Biorad, Hercules). ELISA assays were read on an ELX800 Universal Microplate Reader (Bio-Tek Instrument Inc., Winooski) with λ = 450 nm for signal and 630 nm for background. Western blot was developed on a FluorChem FC2 System version 3.2 (Cell Biosciences, Santa Clara). CellTiter-Blue assays were read on a SpectraMax M5 microplate reader (Molecular Devices, Sunnyvale).

NMR spectra were obtained on a Bruker AV 500 MHz and DRX 500 MHz spectrometers. For ¹H NMR spectra, a relaxation time of 2 seconds was used for small molecules and 30 seconds for polymers. Gel permeation chromatography was conducted on a Shimadzu high performance liquid chromatography (HPLC) system with a refractive index detector RID-10A, one Polymer Laboratories PLgel guard column, and two Polymer Laboratories PLgel 5μm mixed D columns. Eluent was DMF with LiBr (0.1 M) at 40 °C (flow rate: 0.6 mL/min) Calibration was performed using near-monodisperse poly(methyl methacrylate) standards (Polymer Laboratories). UV measurements were performed on a BioMate 5 (Thermo Spectronic) instrument and a NanoDrop 2000 UV-Vis Spectrophotometer (Thermo Scientific). HPLC purification of chain transfer agents was conducted on a Shimadzu HPLC system with a refractive index detector RID-10A and one Luna μm C18(2) column with methanol and water as eluents. The UCLA Pasarow Mass Spectrometry Facility performed mass spectrometry analysis. SDS-PAGE analysis was performed using 10% poly(acylamide) gels (Bio-Rad).